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dc.contributor.authorVilanova, Laura
dc.contributor.authorLópez-Pérez, Mario
dc.contributor.authorBallester, Ana-Rosa
dc.contributor.authorTeixidó, Neus
dc.contributor.authorUsall, Josep
dc.contributor.authorLara, Isabel
dc.contributor.authorViñas, Inmaculada
dc.contributor.authorTorres, Rosario
dc.contributor.authorGonzález-Candelas, Luis
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2019-04-05T14:52:13Z
dc.date.available2019-06-25T09:42:21Z
dc.date.issued2018-05-31
dc.identifier.citationVilanova, Laura, Mario López-Pérez, Ana-Rosa Ballester, Neus Teixidó, Josep Usall, Isabel Lara, Inmaculada Viñas, Rosario Torres, and Luis González-Candelas. 2018. "Differential Contribution Of The Two Major Polygalacturonases From Penicillium Digitatum To Virulence Towards Citrus Fruit". International Journal Of Food Microbiology 282: 16-23. Elsevier BV. doi:10.1016/j.ijfoodmicro.2018.05.031.ca
dc.identifier.issn0168-1605ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/259
dc.description.abstractThe fungus Penicillium digitatum is the causal agent of the citrus green mould, the major postharvest diseases of citrus fruit. Lesions on the surface of infected fruits first appear as soft areas around the inoculation site, due to maceration of fruit. The macerating activity has been associated with pectinases secreted by the fungus during infection. In order to evaluate the contribution to virulence and macerating activity of the two major polygalacturonases (PGs) secreted by P. digitatum, we have obtained and characterized mutants lacking either pg1 or pg2, the genes encoding PG1 and PG2, respectively. Disease incidence of deletants in either gene was not different from that of the parental strain or ectopic transformants. However, disease progressed more slowly in deletants, especially in those lacking the pg2 gene. The lesions originated by the Δpg2 deletants were not as soft and the pH was not as acid as those originated by either the wild type strain or the ectopic transformants. Total PG activity in the macerated tissue was also lower in fruits infected with the Δpg2 deletants. Interestingly, the macerated tissue of oranges infected with Δpg2 deletants showed around 50% reduction in galacturonic acid content with respect to lesions caused by any other strain.ca
dc.format.extent33ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofInternational Journal of Food Microbiologyca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDifferential contribution of the two major polygalacturonases from Penicillium digitatum to virulence towards citrus fruitca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.relation.projectIDMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2011-30519-C03-01/ES/CARACTERIZACION DE LOS MECANISMOS DE VIRULENCIA Y DEFENSA EN LA INTERACCION HONGOFRUTO COMO HERRAMIENTA PARA EL CONTROL DE PENICILLIUM PATOGENOS DE CITRICOS Y DE MANZANAS/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-55802-R/ES/INFECCION DE FRUTOS CITRICOS POR PENICILLIUM DIGITATUM: DEL ESTUDIO DE LOS MECANISMOS DE PATOGENICIDAD Y DEFENSA AL DESARROLLO DE NUEVOS SISTEMAS DE CONTROL/ca
dc.relation.projectIDMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2011-30519-C03-03/ES/CARACTERIZACION DE LOS MECANISMOS DE VIRULENCIA Y DEFENSA EN LA INTERACCION HONGOFRUTO COMO HERRAMIENTA PARA EL CONTROL DE PENICILLIUM PATOGENOS DE CITRICOS Y DE MANZANAS/ca
dc.subject.udc632ca
dc.identifier.doihttps://doi.org/10.1016/j.ijfoodmicro.2018.05.031ca
dc.contributor.groupPostcollitaca


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