Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study
Visualitza/Obre
Autor/a
Kikuti, Mariana
Sanhueza, Juan
Pamornchainavakul, Nakarin
Kevill, Jessica
Yang, My
Paploski, Igor A. D.
Lenskaia, Tatiana
Odogwu, Nkechi M.
Kiehne, Ross
VanderWaal, Kimberly
Schroeder, Declan
Corzo, Cesar A.
Data de publicació
2023-08-30ISSN
1999-4915
Resum
Describing PRRSV whole-genome viral diversity data over time within the host and withinfarm is crucial for a better understanding of viral evolution and its implications. A cohort study was
conducted at one naïve farrow-to-wean farm reporting a PRRSV outbreak. All piglets 3–5 days of age
(DOA) born to mass-exposed sows through live virus inoculation with the recently introduced wildtype virus two weeks prior were sampled and followed up at 17–19 DOA. Samples from 127 piglets
were individually tested for PRRSV by RT-PCR and 100 sequences were generated using Oxford
Nanopore Technologies chemistry. Female piglets had significantly higher median Ct values than
males (15.5 vs. 13.7, Kruskal–Wallis p < 0.001) at 3–5 DOA. A 52.8% mortality between sampling
points was found, and the odds of dying by 17–19 DOA decreased with every one unit increase
in Ct values at 3–5 DOA (OR = 0.76, 95% CI 0.61–0.94, p = 0.01). Although the within-pig percent
nucleotide identity was overall high (99.7%) between 3–5 DOA and 17–19 DOA samples, ORFs 4
and 5a showed much lower identities (97.26% and 98.53%, respectively). When looking solely at
ORF5, 62% of the sequences were identical to the 3–5 DOA consensus. Ten and eight regions showed
increased nucleotide and amino acid genetic diversity, respectively, all found throughout ORFs 2a/2b,
4, 5a/5, 6, and 7.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
11
Publicat per
MDPI
Publicat a
Viruses
Citació
Kikuti, Mariana, Carles Vilalta, Juan Sanhueza, Nakarin Pamornchainavakul, Jessica L. Kevill, Ming‐Chien Yang, Igor Adolfo Dexheimer Paploski, et al. 2023 “Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study.” Viruses 15 (9): 1837. doi:10.3390/v15091837.
Programa
Sanitat Animal
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