Quantification of camelid cytokine mRNA expression in PBMCs by microfluidic qPCR technology
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Author
Te, Nigger
Publication date
2023-09-17ISSN
0145-305X
Abstract
Camelids are economically and socially important in several parts of the world and might carry pathogens with
epizootic or zoonotic potential. However, biological research in these species is limited due to lack of reagents.
Here, we developed RT-qPCR assays to quantify a panel of camelid innate and adaptive immune response genes,
which can be monitored in a single run. The assays were validated with PHA, PMA-ionomycin, and Poly I:Cstimulated PBMCs from alpaca, dromedary camel and llama, including normalization by multiple reference
genes. Further, comparative gene expression analyses for the different camelid species were performed by a
unique microfluidic qPCR assay. Compared to unstimulated controls, PHA and PMA-ionomycin stimulation
elicited robust Th1 and Th2 responses in PBMCs from camelid species. Additional activation of type I and type III
IFN signalling pathways was described exclusively in PHA-stimulated dromedary lymphocytes, in contrast to
those from alpaca and llama. We also found that PolyI:C stimulation induced robust antiviral response genes in
alpaca PBMCs. The proposed methodology should be useful for the measurement of immune responses to
infection or vaccination in camelid species.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
44
Publisher
Elsevier
Is part of
Developmental and Comparative Immunology
Citation
Rodón, Jordi, Nigeer Te, María Ballester, Joaquím Segalés, Júlia Vergara‐Alert, and Albert Bensaïd. 2023. “Quantification of Camelid Cytokine mRNA Expression in PBMCs by Microfluidic qPCR Technology.” Developmental and Comparative Immunology 149: 105061. doi:10.1016/j.dci.2023.105061.
Grant agreement number
EC/PF7/115760/EU/Zoonotic Anticipation and Preparedness Initiative/ZAPI
EC/H2020/731014/EU/Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation/VetBioNet
Program
Genètica i Millora Animal
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/