First identification and characterization of ovine gammaherpesvirus type 2 in horses and artiodactyla from an outbreak of malignant catarrhal fever in Mexico
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Author
Madrigal-Valencia, Tania Lucia
Saavedra-Montañez, Manuel
Pérez-Torres, Armando
Hernández, Jesús
Domínguez Hernández, Yesmín
Candanosa-Aranda, Irma Eugenia
Pérez-Guiot, Alfredo
Ramírez-Mendoza, Humberto
Publication date
2023-09-01ISSN
1932-6203
Abstract
Ovine gammaherpesvirus 2 (OvHV-2), a member of the genus Macavirus, causes sheepassociated malignant catarrhal fever (SA-MCF), a fatal lymphoproliferative disease affecting
a wide variety of ungulates in addition to horses. This study described an outbreak of SAMCF in Mexico and the identification of the OvHV-2 virus in primary rabbit testis cultures
through the generation of intranuclear inclusion bodies, syncytia, immunofluorescence (IF),
immunocytochemistry (ICC), immunohistochemistry (IHC), endpoint polymerase chain
reaction (PCR), and partial sequencing of the ORF75 gene. The animals involved in this outbreak showed mucogingival ulcers in the vestibule of the mouth and tongue, hypersalivation, corneal opacity, reduced food consumption, and weight loss of variable severity. These
clinical signs and the histopathological findings suggested the diagnosis of SA-MCF. Buffy
coat fractions from the anticoagulated blood samples of ill animals were collected and analyzed by PCR. Positive buffy coats were used to inoculate the primary cell cultures of rabbit
testis to identify the virus. Small clusters of refractile cytomegalic cells, characteristic of viral
cytopathic effects, were observed between 48 and 72 h post-infection. Furthermore, intranuclear acidophilic inclusion bodies (IBs) were identified in the inoculated primary culture
cells, and the cytoplasm showed immunoreactivity with hyperimmune rabbit serum against
OvHV-2. Moreover, in the liver histological sections from sick deer, immunoreactive juxtanuclear IBs were identified with the same rabbit hyperimmune serum. The obtained
sequences were aligned with the OvHV-2 sequences reported in GenBank and revealed a
nucleotide identity higher than 98%. Based on the evidence provided in this study, we conclude that the outbreak of SA-MCF in the municipality of Tequisquiapan in the state of Queretaro, Mexico, was caused by OvHV-2. This is the second study reporting that horses are
susceptible to OvHV-2 infection and can develop SA-MCF. We identified for the first time in
Mexico, the presence of OvHV-2 in buffy coats from horses and Artiodactyla.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
23
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Madrigal-Valencia, Tania Lucia, Manuel Saavedra‐Montañez, Armando Pérez‐Torres, Jesús Hernández, Joaquím Segalés, Yesmín Domínguez Hernández, Irma Eugenia Candanosa-Aranda, Alfredo Pérez-Guiot, and Humberto Ramírez‐Mendoza. 2023. “First Identification and Characterization of Ovine Gammaherpesvirus Type 2 in Horses and Artiodactyla from an Outbreak of Malignant Catarrhal Fever in Mexico.” PLOS ONE 18 (9): e0290309. doi:10.1371/journal.pone.0290309.
Program
Sanitat Animal
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- ARTICLES CIENTÍFICS [2555]
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