Fermentation of Plant-Based Feeds with Lactobacillus acidophilus Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (Oreochromis niloticus) Reared in a Biofloc System
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Author
Neves, Nataly Oliveira Dos Santos
De Dea Lindner, Juliano
Stockhausen, Larissa
Delziovo, Fernanda Regina
Bender, Mariana
Serzedello, Letícia
Cipriani, Luiz Augusto
Ha, Natalia
Skoronski, Everton
El Hadi Perez Fabregat, Thiago
Publication date
2024-01-21ISSN
2076-2615
Abstract
This study evaluated the effect of fermentation with Lactobacillus acidophilus on the biochemical and nutritional compositions of a plant-based diet and its effects on the productive performance and intestinal health of juvenile Nile tilapia (Oreochromis niloticus) reared in a biofloc technology (BFT) system. The in vitro kinetics of feed fermentation were studied to determine the L. acidophilus growth and acidification curve through counting the colony-forming units (CFUs) mL−1 and measuring the pH. Physicochemical and bromatological analyses of the feed were also performed. Based on the microbial growth kinetics results, vegetable-based Nile tilapia feeds fermented for 6 (FPB6) and 18 (FPB18) h were evaluated for 60 days. Fermented diets were compared with a positive control diet containing fishmeal (CFM) and a negative control diet without animal protein (CPB). Fermentation with L. acidophilus increased lactic acid bacteria (LAB) count and the soluble protein concentration of the plant-based feed, as well as decreasing the pH (p < 0.05). FPB treatments improved fish survival compared with CPB (p < 0.05). Fermentation increased feed intake but worsened feed efficiency (p < 0.05). The use of fermented feeds increased the LAB count and reduced pathogenic bacteria both in the BFT system’s water and in the animals’ intestines (p < 0.05). Fermented plant-based feeds showed greater villi (FPB6; FPB18) and higher goblet cell (FPB6) counts relative to the non-fermented plant-based feed, which may indicate improved intestinal health. The results obtained in this study are promising and show the sustainable potential of using fermented plant-based feeds in fish feeding rather than animal protein and, in particular, fishmeal.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
637 - Produce of domestic (farmyard) animals and game
Pages
22
Publisher
MDPI
Is part of
Animals
Citation
Oliveira Dos Santos Neves, Nataly, Juliano De Dea Lindner, Larissa Stockhausen, Fernanda Regina Delziovo, Mariana Bender, Letícia Serzedello, Luiz Augusto Cipriani, et al. 2024. “Fermentation of Plant-Based Feeds with Lactobacillus Acidophilus Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (Oreochromis Niloticus) Reared in a Biofloc System.” Animals 14 (2): 332. https://doi.org/10.3390/ani14020332.
Grant agreement number
MICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/FJC2020-043933-I/ES/ /
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/