Safety and Efficacy upon Infection in Sheep with Rift Valley Fever Virus ZH548-rA2, a Triple Mutant Rescued Virus
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Author
Moreno, Sandra
Lorenzo, Gema
de la Losa, Nuria
Alonso, Celia
Charro, Elena
Sánchez-Cordón, Pedro J.
Borrego, Belén
Brun, Alejandro
Publication date
2024-01-05ISSN
1999-4915
Abstract
The introduction of three single nucleotide mutations into the genome of the virulent RVFV
ZH548 strain allows for the rescue of a fully attenuated virus in mice (ZH548-rA2). These mutations
are located in the viral genes encoding the RdRp and the non-structural protein NSs. This paper
shows the results obtained after the subcutaneous inoculation of ZH548-rA2 in adult sheep and the
subsequent challenge with the parental virus (ZH548-rC1). Inoculation with the ZH548-rA2 virus
caused no detectable clinical or pathological effect in sheep, whereas inoculation of the parental
rC1 virus caused lesions compatible with viral infection characterised by the presence of scattered
hepatic necrosis. Viral infection was confirmed via immunohistochemistry, with hepatocytes within
the necrotic foci appearing as the main cells immunolabelled against viral antigen. Furthermore,
the inoculation of sheep with the rA2 virus prevented the liver damage expected after rC1 virus
inoculation, suggesting a protective efficacy in sheep which correlated with the induction of both
humoral and cell-mediated immune responses.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
14
Publisher
MDPI
Is part of
Viruses
Citation
Moreno, Sandra, Gema Lorenzo, Álvaro López-Valiñas, Nuria De La Losa, Celia Alonso, Elena Charro, José I. Núñez, P.J. Sánchez-Cordón, Belén Borrego, and Alejandro Brun. 2024. “Safety and Efficacy upon Infection in Sheep with Rift Valley Fever Virus ZH548-rA2, a Triple Mutant Rescued Virus.” Viruses 16 (1): 87. doi:10.3390/v16010087.
Grant agreement number
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-83226-R/ES/UTILIZACION DEL VIRUS DE LA FIEBRE DEL VALLE DEL RIFT COMO VECTOR VACUNAL: MEJORA RACIONAL DE SU SEGURIDAD, ESTABILIDAD Y TRAZABILIDAD/
FEDER/ / /EU/ /
MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-122567OB-I00/ES/DISEÑO DE UNA PLATAFORMA VACUNAL MULTIVALENTE BASADA EN LA VARIANTE 40FP8 DEL VIRUS DE LA FIEBRE DEL VALLE DEL RIFT/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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