Ppe.RPT/SSC‑1: from QTL mapping to a predictive KASP test for ripening time and soluble solids concentration in peach
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Author
da Silva Linge, Cassia
Fu, Wanfang
Rawadoozi, Zena
Cai, Lichun
Byrne, David H.
Worthington, Margaret
Gasic, Ksenija
Publication date
2024-01-17ISSN
2045-2322
Abstract
Genomic regions associated with ripening time (RPT) and soluble solids concentration (SSC) were
mapped using a pedigreed population including multiple F1 and F2 families from the Clemson
University peach breeding program (CUPBP). RPT and SSC QTLs were consistently identifed in two
seasons (2011 and 2012) and the average datasets (average of two seasons). A target region spanning
10,981,971–11,298,736 bp on chromosome 4 of peach reference genome used for haplotype analysis
revealed four haplotypes with signifcant diferences in trait values among diferent diplotype
combinations. Favorable alleles at the target region for both RPT and SSC were determined and a
DNA test for predicting RPT and SSC was developed. Two Kompetitive Allele Specifc PCR (KASP)
assays were validated on 84 peach cultivars and 163 seedlings from the CUPBP, with only one assay
(Ppe.RPT/SSC‑1) needed to predict between early and late-season ripening cultivars and low and
high SSC. These results advance our understanding of the genetic basis of RPT and SSC and facilitate
selection of new peach cultivars with the desired RPT and SSC
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
13
Publisher
Nature Research
Is part of
Scientific Reports
Citation
Da Silva Linge, Cássia, Wanfang Fu, Alejandro Calle, Zena Rawandoozi, Lichun Cai, David Byrne, Margaret Worthington, and Ksenija Gašić. 2024. “Ppe.RPT/SSC-1: From QTL Mapping to a Predictive KASP Test For Ripening Time And Soluble Solids Concentration in Peach.” Scientific Reports 14 (1). https://doi.org/10.1038/s41598-024-51599-2.
Program
Fructicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2651]
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