Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus
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Publication date
2012-04-19ISSN
1932-6203
Abstract
In this study we propose a novel bacterial vaccine strategy where non-pathogenic bacteria are complemented with traits
desirable for the induction of protective immunity. To illustrate the proof of principle of this novel vaccination strategy, we
use the model organism of intracellular immunity Listeria. We introduced a, low copy number BAC-plasmid harbouring the
virulence gene cluster (vgc) of L. monocytogenes (Lm) into the non-pathogenic L. innocua (L.inn) strain and examined for its
ability to induce protective cellular immunity. The resulting strain (L.inn::vgc) was attenuated for virulence in vivo and
showed a strongly reduced host detrimental inflammatory response compared to Lm. Like Lm, L.inn::vgc induced the
production of Type I Interferon’s and protection was mediated by Listeria-specific CD8+ T cells. Rational vaccine design
whereby avirulent strains are equipped with the capabilities to induce protection but lack detrimental inflammatory effects
offer great promise towards future studies using non-pathogenic bacteria as vectors for vaccination.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
9
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Mohamed, Walid, Shneh Sethi, Svetlin Tchatalbachev, Ayub Darji, and Trinad Chakraborty. 2012. “Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria Innocua Harboring the VGC Locus.” PloS One 7 (4): e35503. doi:10.1371/journal.pone.0035503.
Program
Sanitat Animal
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- ARTICLES CIENTÍFICS [2555]
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