Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection
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Author
Díaz, Ivan
Gimeno, Mariona
Darwich, Laila
Navarro, Nuria
Kuzemtseva, Liudmila
López, Sergio
Galindo, Ivan
Segalés, Joaquim
Martín, Margarita
Mateu, Enric
Publication date
2012-04-19ISSN
0928-4249
Abstract
The present study characterized the homologous and heterologous immune response in type-I porcine
reproductive and respiratory syndrome virus (PRRSV) infection. Two experiments were conducted: in experiment 1,
eight pigs were inoculated with PRRSV strain 3262 and 84 days post-inoculation (dpi) they were challenged with
either strain 3262 or strain 3267 and followed for the next 14 days (98 dpi). In experiment 2, eight pigs were
inoculated with strain 3267 and challenged at 84 dpi as above. Clinical course, viremia, humoral response
(neutralizing and non-neutralizing antibodies, NA) and virus-specific IFN-γ responses (ELISPOT) were evaluated all
throughout the study. Serum levels of IL-1, IL-6, IL-8, TNF-α and TGF-β were determined (ELISA) after the second
challenge. In experiment 1 primo-inoculation with strain 3262 induced viremia of ≤ 28 days, low titres of
homologous NA but strong IFN-γ responses. In contrast, strain 3267 induced longer viremias (up to 56 days), higher
NA titres (≤ 6 log2) and lower IFN-γ responses. Inoculation with 3267 produced higher serum IL-8 levels. After the
re-challenge at 84 dpi, pigs in experiment 1 developed mostly a one week viremia regardless of the strain used. In
experiment 2, neither the homologous nor the heterologous challenge resulted in detectable viremia although
PRRSV was present in tonsils of some animals. Homologous re-inoculation with 3267 produced elevated TGF-β
levels in serum for 7–14 days but this did not occur with the heterologous re-inoculation. In conclusion, inoculation
with different PRRSV strains result in different virological and immunological outcomes and in different degrees of
homologous and heterologous protection.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
15
Publisher
BMC
Is part of
Veterinary Research
Citation
Díaz, Iván, Mariona Gimeno, Laila Darwich, Núria Navarro, Liudmila Kuzemtseva, Sergio Luis González López, Iván Galindo, et al. 2012. “Characterization of Homologous and Heterologous Adaptive Immune Responses in Porcine Reproductive and Respiratory Syndrome Virus Infection.” Veterinary Research 43 (1): 30. doi:10.1186/1297-9716-43-30.
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2008-05708-C02-02/ES/CARACTERIZACION ANTIGENICA DE CEPAS DEL VIRUS DEL SINDROME REPRODUCTOR Y RESPIRATORIO PORCINO DE DISTINTO ORIGEN Y SU RELEVANCIA PARA EL DESARROLLO DE VACUNAS EFICACES/GAN
Program
Sanitat Animal
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- ARTICLES CIENTÍFICS [2555]
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