Mostrar el registro sencillo del ítem
Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
dc.contributor.author | Campàs, Mònica | |
dc.contributor.author | Reverté, Jaume | |
dc.contributor.author | Tudó, Àngels | ca |
dc.contributor.author | Alkassar, Mounira | ca |
dc.contributor.author | Diogène, Jorge | ca |
dc.contributor.author | Sureda, Francesc X. | ca |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2024-04-26T09:00:40Z | |
dc.date.available | 2024-04-26T09:00:40Z | |
dc.date.issued | 2024-04-15 | |
dc.identifier.citation | Campàs, Mònica, Jaume Reverté, Àngels Tudó, Mounira Alkassar, Jorge Diogène, and Francesc X. Sureda. 2024. “Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples.” Marine Drugs 22 (4): 176. https://doi.org/10.3390/md22040176. | ca |
dc.identifier.issn | 1660-3397 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/2950 | |
dc.description.abstract | Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simple methods for its detection and quantification. In this work, we describe the use of an automated patch clamp (APC) system with Neuro-2a cells for the determination of TTX contents in pufferfish samples. The cells showed an IC50 of 6.4 nM for TTX and were not affected by the presence of muscle, skin, liver, and gonad tissues of a Sphoeroides pachygaster specimen (TTX-free) when analysed at 10 mg/mL. The LOD achieved with this technique was 0.05 mg TTX equiv./kg, which is far below the Japanese regulatory limit of 2 mg TTX equiv./kg. The APC system was applied to the analysis of extracts of a Lagocephalus sceleratus specimen, showing TTX contents that followed the trend of gonads > liver > skin > muscle. The APC system, providing an in vitro toxicological approach, offers the advantages of being sensitive, rapid, and reliable for the detection of TTX-like compounds in seafood. | ca |
dc.description.sponsorship | This research was funded by the Ministerio de Ciencia e Innovación (MICIN) and the Agencia Estatal de Investigación (AEI) (Spain) through the CELLECTRA (PID2020-112976RB-C21 and PID2020-112976RB-C22) project. J.R. acknowledges IRTA for his Ph.D. grant (CPI0422). A.T. acknowledges Ministerio de Universidades (Spain) for her Margarita Salas postdoc grant (2021URV-MS-24). M.A. acknowledges MICIN and AEI for her Ph.D. grant (PRE2019-088181). Authors also acknowledge the support from the CERCA Program/Generalitat de Catalunya. The article processing fees were funded by MDPI. | ca |
dc.format.extent | 14 | ca |
dc.language.iso | eng | ca |
dc.publisher | MDPI | ca |
dc.relation.ispartof | Marine Drugs | ca |
dc.rights | Attribution 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA | ca |
dc.relation.projectID | MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA | ca |
dc.subject.udc | 574 | ca |
dc.identifier.doi | https://doi.org/10.3390/md22040176 | ca |
dc.contributor.group | Aigües Marines i Continentals | ca |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
ARTICLES CIENTÍFICS [2.838]