Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A
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Author
Fernández, Ignacio
Mazurais, David
Zambonino-Infante, Jose Luís
Publication date
2011-02-09ISSN
1471-213X
Abstract
Background: Vitamin A (VA) has a key role in vertebrate morphogenesis, determining body patterning and growth
through the control of cell proliferation and differentiation processes. VA regulates primary molecular pathways of
those processes by the binding of its active metabolite (retinoic acid) to two types of specific nuclear receptors:
retinoic acid receptors (RARs) and retinoid X receptors (RXRs), which promote transcription of downstream target
genes. This process is well known in most of higher vertebrates; however, scarce information is available regarding
fishes. Therefore, in order to gain further knowledge of fish larval development and its disruption by nutritional VA
imbalance, the relative expression of some RARs and RXRs, as well as several genes involved in morpho- and
skeletogenesis such as peroxisome proliferator-activated receptors (PPARA, PPARB and PPARG); retinol-binding
protein (RBP); insulin-like growth factors I and II (IGF1 and IGF2, respectively); bone morphogenetic protein 2
(Bmp2); transforming growth factor b-1 (TGFB1); and genes encoding different extracellular matrix (ECM) proteins
such as matrix Gla protein (mgp), osteocalcin (bglap), osteopontin (SPP1), secreted protein acidic and rich in
cysteine (SPARC) and type I collagen a1 chain (COL1A1) have been studied in gilthead sea bream.
Results: During gilthead sea bream larval development, specific expression profiles for each gene were tightly
regulated during fish morphogenesis and correlated with specific morphogenetic events and tissue development.
Dietary hypervitaminosis A during early larval development disrupted the normal gene expression profile for genes
involved in RA signalling (RARA), VA homeostasis (RBP) and several genes encoding ECM proteins that are linked to
skeletogenesis, such as bglap and mgp.
Conclusions: Present data reflects the specific gene expression patterns of several genes involved in larval fish RA
signalling and skeletogenesis; and how specific gene disruption induced by a nutritional VA imbalance underlie the
skeletal deformities. Our results are of basic interest for fish VA signalling and point out some of the potential
molecular players involved in fish skeletogenesis. Increased incidences of skeletal deformities in gilthead sea bream
fed with hypervitaminosis A were the likely ultimate consequence of specific gene expression disruption at critical
development stages.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
63 - Agriculture and related sciences and techniques
Pages
20
Publisher
BMC
Is part of
BMC Developmental Biology
Citation
Fernández, Ignacio, María J. Darias, Karl B. Andrée, David Mazurais, José‐Luis Zambonino-Infante, and Enric Gisbert. 2011. “Coordinated Gene Expression During Gilthead Sea Bream Skeletogenesis and Its Disruption by Nutritional Hypervitaminosis A.” BMC Developmental Biology 11 (1): 7. doi: 10.1186/1471-213x-11-7
Grant agreement number
MEC/Programa Nacional de Potenciación de Recursos Humanos/AGL2005-02478/ES/ /
MICINN/Programa Nacional de Internacionalización de la I+D/HF2008-0079/ES/The role of dietary lipids on fish larvae quality: effects on skeletogenesis and pigmentation/
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2008-03897-C04-01/ES/EL PAPEL DE LOS LIPIDOS SOBRE LA CALIDAD DE LARVAS DE PECES: EFECTOS SOBRE DEFORMACIONES ESQUELETICAS Y PROBLEMAS PIGMENARIOS/
Program
Aqüicultura
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- ARTICLES CIENTÍFICS [2594]
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