Mostra el registre parcial de l'element
Mass Spectrometric Detection of Cholesterol Oxidation in Bovine Sperm
dc.contributor.author | Brouwers, Jos F. | |
dc.contributor.author | Boerke, Arjan | |
dc.contributor.author | Silva, Patrícia F.N. | |
dc.contributor.author | Garcia-Gil, Nuria | |
dc.contributor.author | van Gestel, Renske A. | |
dc.contributor.author | Helms, J. Bernd | |
dc.contributor.author | van de Lest, Chris H.A. | |
dc.contributor.author | Gadella, Bart M. | |
dc.contributor.other | Indústries Alimentàries | ca |
dc.date.accessioned | 2024-05-31T09:18:26Z | |
dc.date.available | 2024-05-31T09:18:26Z | |
dc.date.issued | 2011-07-01 | |
dc.identifier.citation | Brouwers, Jos F., Arjan Boerke, Patrícia F.N. Silva, Nuria Garcia-Gil, Renske A. Van Gestel, J. Bernd Helms, Chris H.A. Van De Lest, and Bart M. Gadella. 2011. “Mass Spectrometric Detection of Cholesterol Oxidation in Bovine Sperm.” Biology of Reproduction 85 (1): 128–136. doi:10.1095/biolreprod.111.091207 | ca |
dc.identifier.issn | 0006-3363 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/3014 | |
dc.description.abstract | We report on the presence and formation of cholesterol oxidation products (oxysterols) in bovine sperm. Although cholesterol is the most abundant molecule in the membrane of mammalian cells and is easily oxidized, this is the first report on cholesterol oxidation in sperm membranes as investigated by state-of-the-art liquid chromatographic and mass spectrometric methods. First, oxysterols are already present in fresh semen samples, showing that lipid peroxidation is part of normal sperm physiology. After chromatographic separation (by high-performance liquid chromatography), the detected oxysterol species were identified with atmospheric pressure chemical ionization mass spectrometry in multiple-reaction-monitoring mode that enabled detection in a broad and linear concentration range (0.05–100 pmol for each oxysterol species detected). Second, exposure of living sperm cells to oxidative stress does not result in the same level and composition of oxysterol species compared with oxidative stress imposed on reconstituted vesicles from protein-free sperm lipid extracts. This suggests that living sperm cells protect themselves against elevated oxysterol formation. Third, sperm capacitation induces the formation of oxysterols, and these formed oxysterols are almost completely depleted from the sperm surface by albumin. Fourth, and most importantly, capacitation after freezing/thawing of sperm fails to induce both the formation of oxysterols and the subsequent albumin-dependent depletion of oxysterols from the sperm surface. The possible physiological relevance of capacitationdependent oxysterol formation and depletion at the sperm surface as well as the omission of this after freezing/thawing semen is discussed. | ca |
dc.description.sponsorship | Supported by the research program Biology of Reproductive Cells of the Graduate School of Animal Sciences from the Faculty of Veterinary Medicine of Utrecht University. P.F.N.S. is supported by Portuguese Foundation for Science and Technology, Ministry for Science, Technology, and Higher Education grant SFRH/2888/2000; R.A.v.G. was financed by ZonMw grant 903-44-156; A.B. was financed by the High Potentials program of Utrecht University; and N.G.-G. was funded by Spanish Ministry of Education and Science grant EX 2005- 0460. | ca |
dc.format.extent | 9 | ca |
dc.language.iso | eng | ca |
dc.publisher | Oxford University Press | ca |
dc.relation.ispartof | Biology of Reproduction | ca |
dc.rights | Attribution-NonCommercial 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.title | Mass Spectrometric Detection of Cholesterol Oxidation in Bovine Sperm | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MEC/ /EX2005-0460/ES/ / | ca |
dc.subject.udc | 663/664 | ca |
dc.identifier.doi | https://doi.org/10.1095/biolreprod.111.091207 | ca |
dc.contributor.group | Qualitat i Tecnologia Alimentària | ca |
Fitxers en aquest element
Aquest element apareix en la col·lecció o col·leccions següent(s)
-
ARTICLES CIENTÍFICS [2.694]