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dc.contributor.authorLecours, Marie-Pier
dc.contributor.authorSegura, Mariela
dc.contributor.authorLachance, Claude
dc.contributor.authorMussa, Tufaria
dc.contributor.authorSurprenant, Charles
dc.contributor.authorMontoya, Maria
dc.contributor.authorGottschalk, Marcelo
dc.contributor.otherProducció Animalca
dc.date.accessioned2024-05-31T09:38:54Z
dc.date.available2024-05-31T09:38:54Z
dc.date.issued2011-06-02
dc.identifier.citationLecours, Marie-Pier, Mariela Segura, Claude Lachance, Tufaria Mussa, Charles Surprenant, Maria Montoya, and Marcelo Gottschalk. 2011. “Characterization of Porcine Dendritic Cell Response to Streptococcus Suis.” Veterinary Research 42 (1): 72. doi:10.1186/1297-9716-42-72ca
dc.identifier.issn0928-4249ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/3015
dc.description.abstractStreptococcus suis is a major swine pathogen and important zoonotic agent causing mainly septicemia and meningitis. However, the mechanisms involved in host innate and adaptive immune responses toward S. suis as well as the mechanisms used by S. suis to subvert these responses are unknown. Here, and for the first time, the ability of S. suis to interact with bone marrow-derived swine dendritic cells (DCs) was evaluated. In addition, the role of S. suis capsular polysaccharide in modulation of DC functions was also assessed. Well encapsulated S. suis was relatively resistant to phagocytosis, but it increased the relative expression of Toll-like receptors 2 and 6 and triggered the release of several cytokines by DCs, including IL-1b, IL-6, IL-8, IL-12p40 and TNF-a. The capsular polysaccharide was shown to interfere with DC phagocytosis; however, once internalized, S. suis was readily destroyed by DCs independently of the presence of the capsular polysaccharide. Cell wall components were mainly responsible for DC activation, since the capsular polysaccharide-negative mutant induced higher cytokine levels than the wild-type strain. The capsular polysaccharide also interfered with the expression of the co-stimulatory molecules CD80/86 and MHC-II on DCs. To conclude, our results show for the first time that S. suis interacts with swine origin DCs and suggest that these cells might play a role in the development of host innate and adaptive immunity during an infection with S. suis serotype 2.ca
dc.description.sponsorshipWe acknowledge Dr J. Dominguez (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Spain) for the gift of hybridoma supernatants. We thank D. Montpetit (CRDA, Saint-Hyacinthe, Québec, Canada) for SEM analysis and Dr M. Houde for his collaboration on confocal analysis. This work was supported by Natural Sciences and Engineering Research Council of Canada (NSERC) grant #154280 as well as Discovery Accelerator Supplement #380299 to MG and partially supported by NSERC grant #342150-07 to MS. MS is the recipient of a Fonds de recherche en santé du Québec (FRSQ) Career Award. M-PL is the recipient of Fonds québécois de la recherche sur la nature et les technologies (FQRNT) and NSERC doctoral awards. Part of this work was also supported by FQRNT international stage funding to M-PL and CRIP-FQRNT-New Initiative support to MG and MS.ca
dc.format.extent12ca
dc.language.isoengca
dc.publisherBMCca
dc.relation.ispartofVeterinary Researchca
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleCharacterization of porcine dendritic cell response to Streptococcus suisca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1186/1297-9716-42-72ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/
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