dc.contributor.author | Lecours, Marie-Pier | |
dc.contributor.author | Segura, Mariela | |
dc.contributor.author | Lachance, Claude | |
dc.contributor.author | Mussa, Tufaria | |
dc.contributor.author | Surprenant, Charles | |
dc.contributor.author | Montoya, Maria | |
dc.contributor.author | Gottschalk, Marcelo | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2024-05-31T09:38:54Z | |
dc.date.available | 2024-05-31T09:38:54Z | |
dc.date.issued | 2011-06-02 | |
dc.identifier.citation | Lecours, Marie-Pier, Mariela Segura, Claude Lachance, Tufaria Mussa, Charles Surprenant, Maria Montoya, and Marcelo Gottschalk. 2011. “Characterization of Porcine Dendritic Cell Response to Streptococcus Suis.” Veterinary Research 42 (1): 72. doi:10.1186/1297-9716-42-72 | ca |
dc.identifier.issn | 0928-4249 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/3015 | |
dc.description.abstract | Streptococcus suis is a major swine pathogen and important zoonotic agent causing mainly septicemia and
meningitis. However, the mechanisms involved in host innate and adaptive immune responses toward S. suis as well
as the mechanisms used by S. suis to subvert these responses are unknown. Here, and for the first time, the ability of
S. suis to interact with bone marrow-derived swine dendritic cells (DCs) was evaluated. In addition, the role of S. suis
capsular polysaccharide in modulation of DC functions was also assessed. Well encapsulated S. suis was relatively
resistant to phagocytosis, but it increased the relative expression of Toll-like receptors 2 and 6 and triggered the
release of several cytokines by DCs, including IL-1b, IL-6, IL-8, IL-12p40 and TNF-a. The capsular polysaccharide was
shown to interfere with DC phagocytosis; however, once internalized, S. suis was readily destroyed by DCs
independently of the presence of the capsular polysaccharide. Cell wall components were mainly responsible for DC
activation, since the capsular polysaccharide-negative mutant induced higher cytokine levels than the wild-type
strain. The capsular polysaccharide also interfered with the expression of the co-stimulatory molecules CD80/86 and
MHC-II on DCs. To conclude, our results show for the first time that S. suis interacts with swine origin DCs and
suggest that these cells might play a role in the development of host innate and adaptive immunity during an
infection with S. suis serotype 2. | ca |
dc.description.sponsorship | We acknowledge Dr J. Dominguez (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Spain) for the gift of hybridoma supernatants. We thank D. Montpetit (CRDA, Saint-Hyacinthe, Québec, Canada) for SEM analysis and Dr M. Houde for his collaboration on confocal analysis. This work was supported by Natural Sciences and Engineering Research Council of Canada (NSERC) grant #154280 as well as Discovery Accelerator Supplement #380299 to MG and partially supported by NSERC grant #342150-07 to MS. MS is the recipient of a Fonds de recherche en santé du Québec (FRSQ) Career Award. M-PL is the recipient of Fonds québécois de la recherche sur la nature et les technologies (FQRNT) and NSERC doctoral awards. Part of this work was also supported by FQRNT international stage funding to M-PL and CRIP-FQRNT-New Initiative support to MG and MS. | ca |
dc.format.extent | 12 | ca |
dc.language.iso | eng | ca |
dc.publisher | BMC | ca |
dc.relation.ispartof | Veterinary Research | ca |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Characterization of porcine dendritic cell response to Streptococcus suis | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.1186/1297-9716-42-72 | ca |
dc.contributor.group | Sanitat Animal | ca |