dc.contributor.author | Ampuero Kragten, S. | |
dc.contributor.author | Verkuylen, B. | |
dc.contributor.author | Dahlmans, H. | |
dc.contributor.author | Hortós, M. | |
dc.contributor.author | Garcia-Regueiro, J. A. | |
dc.contributor.author | Dahl, E. | |
dc.contributor.author | Andresen, O. | |
dc.contributor.author | Feitsma, H. | |
dc.contributor.author | Mathur, P. K. | |
dc.contributor.author | Harlizius, B. | |
dc.contributor.other | Indústries Alimentàries | ca |
dc.date.accessioned | 2024-05-31T12:20:55Z | |
dc.date.available | 2024-05-31T12:20:55Z | |
dc.date.issued | 2011-04-26 | |
dc.identifier.citation | Kragten, S. Ampuero, B. Verkuylen, H. Dahlmans, M. Hortos, J. A. Garcia-Regueiro, E. Dahl, O. Andresen, H. Feitsma, P. K. Mathur, and B. Harlizius. 2011. “Inter-laboratory Comparison of Methods to Measure Androstenone in Pork Fat.” Animal 5 (10): 1634–1642. doi:10.1017/s1751731111000553 | ca |
dc.identifier.issn | 1751-7311 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/3020 | |
dc.description.abstract | Today, different analytical methods are used by different laboratories to quantify androstenone in fat tissue. This study shows the
comparison of methods used routinely in different laboratories for androstenone quantification: Time-resolved fluoroimmunoassay
in Norwegian School of Veterinary Science (NSVS; Norway), gas chromatography coupled to mass spectrometry in Co-operative
Central Laboratory (CCL; The Netherlands) and in Institut de Recerca i Tecnologia Agroalimenta `ries (IRTA; Spain), and high-pressure
liquid chromatography in Agroscope Liebefeld-Posieux Research Station (ALP; Switzerland). In a first trial, a set of adipose tissue (AT)
samples from 53 entire males was sent to CCL, IRTA and NSVS for determination of androstenone concentration. The average
androstenone concentration (s.d.) was 2.47 (2.10) mg/g at NSVS, 1.31 (0.98) mg/g at CCL and 0.62 (0.52) mg/g at IRTA. Despite the
large differences in absolute values, inter-laboratory correlations were high, ranging from 0.82 to 0.92. A closer look showed
differences in the preparation step. Indeed, different matrices were used for the analysis: pure fat at NSVS, melted fat at CCL and
AT at IRTA. A second trial was organised in order to circumvent the differences in sample preparation. Back fat samples from 10 entire
males were lyophilised at the ALP labortary in Switzerland and were sent to the other laboratories for androstenone concentration
measurement. The average concentration (s.d.) of androstenone in the freeze-dried AT samples was 0.87 (0.52), 1.03 (0.55), 0.84
(0.46) and 0.99 (0.67) mg/g at NSVS, CCL, IRTA and ALP, respectively, and the pairwise correlations between laboratories ranged from
0.92 to 0.97. Thus, this study shows the influence of the different sample preparation protocols, leading to major differences in the
results, although still allowing high inter-laboratory correlations. The results further highlight the need for method standardisation
and inter-laboratory ring tests for the determination of androstenone. This standardisation is especially relevant when deriving
thresholds of consumer acceptance, whereas the ranking of animals for breeding purposes will be less affected due to the high
correlations between methods | ca |
dc.description.sponsorship | The authors would like to thank TOPIGS for providing the data of entire boars and tissue sampling as well as S. Dubois for the preparation and analysis of the freeze-dried samples at the chemical department of ALP. This research project has been cofinanced by the European Commission, within the 6th Framework Programme, contract no. FOOD-CT-2006-016250 (‘SABRE’). The study represents the authors’ views and does not necessarily represent the position of the Commission, which will not be liable for the use made of such information. | ca |
dc.format.extent | 9 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Animal | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Inter-laboratory comparison of methods to measure androstenone in pork fat | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | EC/FP6/16250/EU/Cutting edge genomics for sustainable animal breeding/SABRE | ca |
dc.subject.udc | 663/664 | ca |
dc.identifier.doi | https://doi.org/10.1017/S1751731111000553 | ca |
dc.contributor.group | Funcionalitat i Seguretat Alimentària | ca |