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dc.contributor.authorAmpuero Kragten, S.
dc.contributor.authorVerkuylen, B.
dc.contributor.authorDahlmans, H.
dc.contributor.authorHortós, M.
dc.contributor.authorGarcia-Regueiro, J. A.
dc.contributor.authorDahl, E.
dc.contributor.authorAndresen, O.
dc.contributor.authorFeitsma, H.
dc.contributor.authorMathur, P. K.
dc.contributor.authorHarlizius, B.
dc.contributor.otherIndústries Alimentàriesca
dc.date.accessioned2024-05-31T12:20:55Z
dc.date.available2024-05-31T12:20:55Z
dc.date.issued2011-04-26
dc.identifier.citationKragten, S. Ampuero, B. Verkuylen, H. Dahlmans, M. Hortos, J. A. Garcia-Regueiro, E. Dahl, O. Andresen, H. Feitsma, P. K. Mathur, and B. Harlizius. 2011. “Inter-laboratory Comparison of Methods to Measure Androstenone in Pork Fat.” Animal 5 (10): 1634–1642. doi:10.1017/s1751731111000553ca
dc.identifier.issn1751-7311ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/3020
dc.description.abstractToday, different analytical methods are used by different laboratories to quantify androstenone in fat tissue. This study shows the comparison of methods used routinely in different laboratories for androstenone quantification: Time-resolved fluoroimmunoassay in Norwegian School of Veterinary Science (NSVS; Norway), gas chromatography coupled to mass spectrometry in Co-operative Central Laboratory (CCL; The Netherlands) and in Institut de Recerca i Tecnologia Agroalimenta `ries (IRTA; Spain), and high-pressure liquid chromatography in Agroscope Liebefeld-Posieux Research Station (ALP; Switzerland). In a first trial, a set of adipose tissue (AT) samples from 53 entire males was sent to CCL, IRTA and NSVS for determination of androstenone concentration. The average androstenone concentration (s.d.) was 2.47 (2.10) mg/g at NSVS, 1.31 (0.98) mg/g at CCL and 0.62 (0.52) mg/g at IRTA. Despite the large differences in absolute values, inter-laboratory correlations were high, ranging from 0.82 to 0.92. A closer look showed differences in the preparation step. Indeed, different matrices were used for the analysis: pure fat at NSVS, melted fat at CCL and AT at IRTA. A second trial was organised in order to circumvent the differences in sample preparation. Back fat samples from 10 entire males were lyophilised at the ALP labortary in Switzerland and were sent to the other laboratories for androstenone concentration measurement. The average concentration (s.d.) of androstenone in the freeze-dried AT samples was 0.87 (0.52), 1.03 (0.55), 0.84 (0.46) and 0.99 (0.67) mg/g at NSVS, CCL, IRTA and ALP, respectively, and the pairwise correlations between laboratories ranged from 0.92 to 0.97. Thus, this study shows the influence of the different sample preparation protocols, leading to major differences in the results, although still allowing high inter-laboratory correlations. The results further highlight the need for method standardisation and inter-laboratory ring tests for the determination of androstenone. This standardisation is especially relevant when deriving thresholds of consumer acceptance, whereas the ranking of animals for breeding purposes will be less affected due to the high correlations between methodsca
dc.description.sponsorshipThe authors would like to thank TOPIGS for providing the data of entire boars and tissue sampling as well as S. Dubois for the preparation and analysis of the freeze-dried samples at the chemical department of ALP. This research project has been cofinanced by the European Commission, within the 6th Framework Programme, contract no. FOOD-CT-2006-016250 (‘SABRE’). The study represents the authors’ views and does not necessarily represent the position of the Commission, which will not be liable for the use made of such information.ca
dc.format.extent9ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofAnimalca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleInter-laboratory comparison of methods to measure androstenone in pork fatca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDEC/FP6/16250/EU/Cutting edge genomics for sustainable animal breeding/SABREca
dc.subject.udc663/664ca
dc.identifier.doihttps://doi.org/10.1017/S1751731111000553ca
dc.contributor.groupFuncionalitat i Seguretat Alimentàriaca


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