A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
View/Open
Author
Diaz, Aurora
Fergany, Mohamed
Formisano, Gelsomina
Ziarsolo, Peio
Blanca, José
Fei, Zhanjun
Staub, Jack E.
Zalapa, Juan E.
Cuevas, Hugo E.
Dace, Gayle
Oliver, Marc
Boissot, Nathalie
Dogimont, Catherine
Pitrat, Michel
Hofstede, René
van Koert, Paul
Harel-Beja, Rotem
Tzuri, Galil
Portnoy, Vitaly
Cohen, Shahar
Schaffer, Arthur
Katzir, Nurit
Xu, Yong
Zhang, Haiying
Fukino, Nobuko
Matsumoto, Satoru
Monforte, Antonio J.
Publication date
2011-07-28ISSN
1471-2229
Abstract
Background: A number of molecular marker linkage maps have been developed for melon (Cucumis melo L.) over
the last two decades. However, these maps were constructed using different marker sets, thus, making
comparative analysis among maps difficult. In order to solve this problem, a consensus genetic map in melon was
constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny,
and comparative quantitative trait loci (QTL) analysis, increasing breeding effectiveness and efficiency via markerassisted selection (MAS).
Results: Under the framework of the International Cucurbit Genomics Initiative (ICuGI, http://www.icugi.org), an
integrated genetic map has been constructed by merging data from eight independent mapping experiments
using a genetically diverse array of parental lines. The consensus map spans 1150 cM across the 12 melon linkage
groups and is composed of 1592 markers (640 SSRs, 330 SNPs, 252 AFLPs, 239 RFLPs, 89 RAPDs, 15 IMAs, 16 indels
and 11 morphological traits) with a mean marker density of 0.72 cM/marker. One hundred and ninety-six of these
markers (157 SSRs, 32 SNPs, 6 indels and 1 RAPD) were newly developed, mapped or provided by industry
representatives as released markers, including 27 SNPs and 5 indels from genes involved in the organic acid
metabolism and transport, and 58 EST-SSRs. Additionally, 85 of 822 SSR markers contributed by Syngenta Seeds
were included in the integrated map. In addition, 370 QTL controlling 62 traits from 18 previously reported
mapping experiments using genetically diverse parental genotypes were also integrated into the consensus map.
Some QTL associated with economically important traits detected in separate studies mapped to similar genomic
positions. For example, independently identified QTL controlling fruit shape were mapped on similar genomic
positions, suggesting that such QTL are possibly responsible for the phenotypic variability observed for this trait in
a broad array of melon germplasm.
Conclusions: Even though relatively unsaturated genetic maps in a diverse set of melon market types have been
published, the integrated saturated map presented herein should be considered the initial reference map for
melon. Most of the mapped markers contained in the reference map are polymorphic in diverse collection of germplasm, and thus are potentially transferrable to a broad array of genetic experimentation (e.g., integration of
physical and genetic maps, colinearity analysis, map-based gene cloning, epistasis dissection, and marker-assisted
selection).
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
575 - General genetics. General cytogenetics
633 - Field crops and their production
Pages
14
Publisher
BMC
Is part of
BMC Plant Biology
Citation
Diaz, Aurora, Mohamed Fergany, Gelsomina Formisano, Peio Ziarsolo, José Blanca, Zhanjun Fei, Jack E Staub, et al. 2011. “A Consensus Linkage Map for Molecular Markers and Quantitative Trait Loci Associated With Economically Important Traits in Melon (Cucumis meloL.).” BMC Plant Biology 11 (1): 111. doi:10.1186/1471-2229-11-111
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2009-12698-C02-02/ES/El Control Genetico De La Forma Del Fruto Y Domesticacion Del Melon En Varias Fuentes De Germoplasma/
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2651]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/