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Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
dc.contributor.author | Chaves, Aida J. | |
dc.contributor.author | Busquets, Núria | |
dc.contributor.author | Valle, Rosa | |
dc.contributor.author | Rivas, Raquel | |
dc.contributor.author | Vergara-Alert, Júlia | |
dc.contributor.author | Dolz, Roser | |
dc.contributor.author | Ramis, Antonio | |
dc.contributor.author | Darji, Ayub | |
dc.contributor.author | Majó, Natàlia | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2024-06-07T10:17:59Z | |
dc.date.available | 2024-06-07T10:17:59Z | |
dc.date.issued | 2011-10-07 | |
dc.identifier.citation | Chaves, Aida J, Núria Busquets, Rosa Valle, Raquel Rivas, Júlia Vergara-Alert, Roser Dolz, Antonio Ramis, Ayub Darji, and Natàlia Majó. 2011. “Neuropathogenesis of a Highly Pathogenic Avian Influenza Virus (H7N1) in Experimentally Infected Chickens.” Veterinary Research 42 (1): 106. doi: 10.1186/1297-9716-42-106 | ca |
dc.identifier.issn | 0928-4249 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/3043 | |
dc.description.abstract | In order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into the central nervous system (CNS) of chickens, specific pathogen free chickens were inoculated with a H7N1 HPAIV. Blood, cerebrospinal fluid (CSF), nasal cavity and brain tissue samples were obtained from 1 to 4 days postinoculation (dpi) of infected and control chickens. Viral antigen topographical distribution, presence of influenza A virus receptors in the brain, as well as, the role of the olfactory route in virus CNS invasion were studied using different immunohistochemistry techniques. Besides, viral RNA load in CSF and blood was quantified by means of a quantitative real-time reverse transcription-polymerase chain reaction. Viral antigen was observed widely distributed in the CNS, showing bilateral and symmetrical distribution in the nuclei of the diencephalon, mesencephalon and rhombencephalon. Viral RNA was detected in blood and CSF at one dpi, indicating that the virus crosses the blood-CSF-barrier early during infection. This early dissemination is possibly favoured by the presence of Siaa2,3 Gal and Siaa2,6 Gal receptors in brain vascular endothelial cells, and Siaa2,3 Gal receptors in ependymal and choroid plexus cells. No viral antigen was observed in olfactory sensory neurons, while the olfactory bulb showed only weak staining, suggesting that the virus did not use this pathway to enter into the brain. The sequence of virus appearance and the topographical distribution of this H7N1 HPAIV indicate that the viral entry occurs via the haematogenous route, with early and generalized spreading through the CSF. | ca |
dc.description.sponsorship | This work was partially supported by the European Project Specific Target Research Project, Euroflu, funded by the sixth Framework Programme (FP6) of the EU (grant 5B-CT-2007-044098). We thank Dr Ana Moreno for kindly providing the avian influenza virus A/Chicken/Italy/5093/9 H7N1 strain. Aida J Chaves is supported by the Programme Alban, the European Union Programme of High Level Scholarships for Latin America, scholarship No. E07D400404CR. The authors greatly appreciate the help provided by Dr Enric Vidal for critically reading the manuscript. | ca |
dc.format.extent | 12 | ca |
dc.language.iso | eng | ca |
dc.publisher | BMC | ca |
dc.relation.ispartof | Veterinary Research | ca |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | EC/FP6/44098/EU/Molecular factors and mechanisms of transmission and pathogenicity of highly pathogenic Avian Influenza Virus/EUROFLU | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.1186/1297-9716-42-106 | ca |
dc.contributor.group | Sanitat Animal | ca |
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