An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
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Author
Mascarell-Creus, Albert
Cañizares, Joaquin
Vilarrasa-Blasi, Josep
Mora-García, Santiago
Blanca, José
Gonzalez-Ibeas, Daniel
Saladié, Montserrat
Roig, Cristina
Deleu, Wim
Picó-Silvent, Belén
López-Bigas, Nuria
Aranda, Miguel A
Nuez, Fernando
Puigdomènech, Pere
Caño-Delgado, Ana I
Publication date
2009-10-12ISSN
1471-2164
Abstract
Background: Melon (Cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the
Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx.
450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade.
However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of
designing new tools for high-throughput analysis of gene expression.
Results: We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from
33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during
interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of
global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant
cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with
functional-enrichment analysis are presented in this study.
Conclusion: The platform validation and enrichment analyses shown in our study indicate that this oligo-based
microarray is amenable for future genetic and functional genomic studies of a wide range of experimental
conditions in melon.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
575 - General genetics. General cytogenetics
633 - Field crops and their production
Pages
15
Publisher
BMC
Is part of
BMC Genomics
Citation
Mascarell-Creus, Albert, Joaquín Cañizares, Josep Vilarrasa-Blasi, Santiago Mora-García, José M. Blanca, Daniel González-Ibeas, Montserrat Saladié, et al. 2009. “An Oligo-based Microarray Offers Novel Transcriptomic Approaches For The Analysis Of Pathogen Resistance And Fruit Quality Traits In Melon (Cucumis Melo L.)”. BMC Genomics 10 (1): 467. doi: 10.1186/1471-2164-10-467
Grant agreement number
MEC/ /GEN2003-20237-C06/ES/ /
MEC/Programa nacional de recursos y tecnologías agroalimentarias/AGL2006-08069/ES/Dianas para la mejora de las resistencias del melón a virus de importancia agronómica/AGR
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2694]
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