Confirmation of Pinnatoxins and Spirolides in Shellfish and Passive Samplers from Catalonia (Spain) by Liquid Chromatography Coupled with Triple Quadrupole and High-Resolution Hybrid Tandem Mass Spectrometry
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Author
García-Altares, María
Casanova, Alexis
Bane, Vaishali
Furey, Ambrose
de la Iglesia, Pablo
Publication date
2014-06-23ISSN
1660-3397
Abstract
Cyclic imines are lipophilic marine toxins that bioaccumulate in seafood. Their structure comprises a cyclic-imino moiety, responsible for acute neurotoxicity in mice. Cyclic imines have not been linked yet to human poisonings and are not regulated in Europe, although the European Food Safety Authority requires more data to perform a conclusive risk assessment for consumers. This work presents the first detection of pinnatoxin G (PnTX-G) in Spain and 13-desmethyl spirolide C (SPX-1) in shellfish from Catalonia (Spain, NW Mediterranean Sea). Cyclic imines were found at low concentrations (2 to 60 µg/kg) in 13 samples of mussels and oysters (22 samples analyzed). Pinnatoxin G has been also detected in 17 seawater samples (out of 34) using solid phase adsorption toxin tracking devices (0.3 to
0.9 µg/kg-resin). Pinnatoxin G and SPX-1 were confirmed with both low and high resolution (<2 ppm) mass spectrometry by comparison of the response with that from reference standards. For other analogs without reference standards, we applied a strategy combining low resolution MS with a triple quadrupole mass analyzer for a fast and reliable screening, and high resolution MS LTQ Orbitrap® for unambiguous confirmation. The advantages and limitations of using high resolution MS without reference standards were discussed.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
27
Publisher
MDPI
Is part of
Marine Drugs
Citation
García-Altares, María, Alexis Casanova, Vaishali Bane, Jorge Diogène, Ambrose Furey, and Pablo de la Iglesia. 2014. "Confirmation Of Pinnatoxins And Spirolides In Shellfish And Passive Samplers From Catalonia (Spain) By Liquid Chromatography Coupled With Triple Quadrupole And High-Resolution Hybrid Tandem Mass Spectrometry". Marine Drugs 12 (6): 3706-3732. doi:10.3390/md12063706.
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/RTA2009-00127-00-00/ES/Importancia de las toxinas marinas en los productos de la pesca y la acuicultura en Cataluña: evaluación del riesgo y propuestas para su gestión/
FEDER/ / /EU/ /
EC/ /SOE1-P1-E129/EU/Promotion of innovation and constitution of stable cooperation networks in technological matters/ALARMTOX
EC/ / /EU/Knowledge Transfer Network for Prevention of Mental Diseases and Cancer in the Atlantic Area/PHARMATLANTIC
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2802]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/