Differences in Muscle Transcriptome among Pigs Phenotypically Extreme for Fatty Acid Composition
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Author
Puig-Oliveras, Anna
Ramayo-Caldas, Yuliaxis
Corominas, Jordi
Estelle, Jordi
Pérez-Montarelo, Dafne
Hudson, Nicholas J.
Folch, Josep M.
Ballester, Maria
Publication date
2014-06-13ISSN
1932-6203
Abstract
Background: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed genes between two groups of 6 sows belonging to an Iberian 6 Landrace backcross with extreme phenotypes according to FA profile. Results: We sequenced the muscle transcriptome acquiring 787.5 M of 75 bp paired-end reads. About 85.1% of reads were mapped to the reference genome. Of the total reads, 79.1% were located in exons, 6.0% in introns and 14.9% in intergenic regions, indicating expressed regions not annotated in the reference genome. We identified a 34.5% of the intergenic regions as interspersed repetitive regions. We predicted a total of 2,372 putative proteins. Pathway analysis with 131
differentially-expressed genes revealed that the most statistically-significant metabolic pathways were related with lipid metabolism. Moreover, 18 of the differentially-expressed genes were located in genomic regions associated with IMF composition in an independent GWAS study in the same genetic background. Thus, our results indicate that the lipid metabolism of FAs is differently modulated when the FA composition in muscle differs. For instance, a high content of PUFA may reduce FA and glucose uptake resulting in an inhibition of the lipogenesis. These results are consistent with previous studies of our group analysing the liver and the adipose tissue transcriptomes providing a view of each of the main organs involved in lipid metabolism. Conclusions: The results obtained in the muscle transcriptome analysis increase the knowledge of the gene regulation of IMF deposition, FA profile and meat quality, in terms of taste and nutritional value. Besides, our results may be important in terms of human health.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
575 - General genetics. General cytogenetics
Pages
11
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Puig-Oliveras, Anna, Yuliaxis Ramayo-Caldas, Jordi Corominas, Jordi Estellé, Dafne Pérez-Montarelo, Nicholas J. Hudson, Joaquim Casellas, Josep M. Folch, and Maria Ballester. 2014. "Differences In Muscle Transcriptome Among Pigs Phenotypically Extreme For Fatty Acid Composition". Plos ONE 9 (6): e99720. doi:10.1371/journal.pone.0099720.
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2011-29821-C02/ES/APLICACION DE METODOS DE SECUENCIACION PARALELA MASIVA Y GENOMICA AL ESTUDIO DE VARIANTES GENICAS QUE REGULAN: CRECIMIENTO, CONFORMACION Y CALIDAD DE CARNE EN CERDO.SUBPROYECTO2/
MEC/Programa nacional de medios de transporte/CSD2007-00036/ES/Centro de Genómica Básica y de orientación Agroalimentaria/
Program
Genètica i Millora Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2802]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/