dc.contributor.author | Cabrera, Antonio | |
dc.contributor.author | Kozik, Alex | |
dc.contributor.author | Howad, Werner | |
dc.contributor.author | Arús, Pere | |
dc.contributor.author | Iezzoni, Amy F | |
dc.contributor.author | van der Knaap, Esther | |
dc.contributor.other | Producció Vegetal | ca |
dc.date.accessioned | 2024-09-18T06:58:37Z | |
dc.date.available | 2024-09-18T06:58:37Z | |
dc.date.issued | 2009-11-29 | |
dc.identifier.citation | Cabrera, Antonio, Alex Kozik, Werner Howad, Pere Arus, Amy F Iezzoni, and Esther Van Der Knaap. 2009. “Development and Bin Mapping of a Rosaceae Conserved Ortholog Set (COS) of Markers.” BMC Genomics 10 (1): 562. doi: 10.1186/1471-2164-10-562 | ca |
dc.identifier.issn | 1471-2164 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/3268 | |
dc.description.abstract | Background: Detailed comparative genome analyses within the economically important Rosaceae
family have not been conducted. This is largely due to the lack of conserved gene-based molecular
markers that are transferable among the important crop genera within the family [e.g. Malus
(apple), Fragaria (strawberry), and Prunus (peach, cherry, apricot and almond)]. The lack of
molecular markers and comparative whole genome sequence analysis for this family severely
hampers crop improvement efforts as well as QTL confirmation and validation studies.
Results: We identified a set of 3,818 rosaceaous unigenes comprised of two or more ESTs that
correspond to single copy Arabidopsis genes. From this Rosaceae Conserved Orthologous Set
(RosCOS), 1039 were selected from which 857 were used for the development of intron-flanking
primers and allele amplification. This led to successful amplification and subsequent mapping of 613
RosCOS onto the Prunus TxE reference map resulting in a genome-wide coverage of 0.67 to 1.06
gene-based markers per cM per linkage group. Furthermore, the RosCOS primers showed
amplification success rates from 23 to 100% across the family indicating that a substantial part of
the RosCOS primers can be directly employed in other less studied rosaceaous crops.
Comparisons of the genetic map positions of the RosCOS with the physical locations of the
orthologs in the Populus trichocarpa genome identified regions of colinearity between the genomes
of Prunus-Rosaceae and Populus-Salicaceae.
Conclusion: Conserved orthologous genes are extremely useful for the analysis of genome
evolution among closely and distantly related species. The results presented in this study
demonstrate the considerable potential of the mapped Prunus RosCOS for genome-wide marker
employment and comparative whole genome studies within the Rosaceae family. Moreover, these
markers will also function as useful anchor points for the genome sequencing efforts currently
ongoing in this family as well as for comparative QTL analyses. | ca |
dc.description.sponsorship | This work is supported by USDA-NRI grants 2008-02259 and 2005-00743. AC was also supported by funds from the Department of Horticulture and Crop Science, The Ohio State University. The authors would like to thank Drs. Dan Sargent and David Chagne for providing DNAs from strawberry and apple, respectively. | ca |
dc.format.extent | 12 | ca |
dc.language.iso | eng | ca |
dc.publisher | BMC | ca |
dc.relation.ispartof | BMC Genomics | ca |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Development and bin mapping of a Rosaceae Conserved Ortholog Set (COS) of markers | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 575 | ca |
dc.subject.udc | 633 | ca |
dc.identifier.doi | https://doi.org/10.1186/1471-2164-10-562 | ca |
dc.contributor.group | Genòmica i Biotecnologia | ca |