First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
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Author
Ferreres, Laura
Sureda, Francesc X.
Publication date
2024-08-08ISSN
0045-6535
Abstract
Pufferfish is one of the most poisonous marine organisms, responsible for numerous poisoning incidents and some human fatalities due to its capability to accumulate potent neurotoxins such as tetrodotoxins (TTXs) and paralytic shellfish toxins (PSTs). In this study, tissue extracts (muscle, skin, liver, intestinal tract and gonads) obtained from sixteen pufferfish specimens of the Lagocephalus lagocephalus and Sphoeroides pachygaster species, collected along the Spanish Mediterranean coast, were analysed for the presence of voltage-gated sodium channel (also known as Nav channel) blockers using cell-based assay (CBA) and automated patch clamp (APC). No toxicity was observed in any of the S. pachygaster specimens, but toxicity was detected in the liver of most L. lagocephalus specimens. Instrumental analysis of these specimens, as well as in one Lagocephalus sceleratus specimen, by high-performance liquid chromatography coupled to fluorescence detection (HPLC-FLD) was performed, which confirmed the presence of PSTs only in L. lagocephalus specimens. This analysis reported the presence of saxitoxin (STX) and decarbamoylsaxitoxin (dcSTX) in all positive samples, being dcSTX the major analogue. These results demonstrate the ability of this species to accumulate PSTs, being the first report of the presence of PSTs in Mediterranean L. lagocephalus specimens. Furthermore, the presence of high PSTs contents in all five tested tissues of one L. lagocephalus specimen pointed the risk that the presence of this toxic fish in the Mediterranean Sea may represent for seafood safety and human health in case of accidental consumption.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
33
Publisher
Elsevier
Is part of
Chemosphere
Citation
Alkassar, Mounira, Àngels Tudó, Maria Rambla-Alegre, Laura Ferreres, Jorge Diogène, Francesc X Sureda, and Mònica Campàs. 2024. “First Record of Paralytic Shellfish Toxins in Marine Pufferfish From the Spanish Mediterranean Coast Using Cell-based Assay, Automated Patch Clamp and HPLC-FLD.” Chemosphere 364: 143053. https://doi.org/10.1016/j.chemosphere.2024.143053.
Grant agreement number
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/ES/PID2023-149899OB-C21/ES/Tecnologías bio-analíticas basadas en anticuerpos, células y espectrometría de masas para explorar las fronteras de las toxinas marinas/BiOCEANsing
MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/ES/PID2023-149899OB-C22/ES/Tecnologías bio-analíticas basadas en pequeñas proteínas combinatorias, canales iónicos artificiales y células para explorar las fronteras de las toxinas marinas/BiOCEANsing
Program
Aigües Marines i Continentals
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- ARTICLES CIENTÍFICS [2850]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/