Expression analysis of candidate genes for fatty acid composition in adipose tissue and identifcation of regulatory regions
Fernández, Ana I.
Folch, Josep M.
The aim of this work was to study the genetic basis of the backfat expression of lipid-related genes associated with meat quality traits in pigs. We performed a genome-wide association study with the backfat gene expression measured in 44 genes by qPCR and the PorcineSNP60 BeadChip genotypes in 115 Iberian x Landrace backcross animals. A total of 193 expression-associated SNPs located in 19 chromosomal regions were associated with expression levels of ACSM5, ELOVL6, FABP4, FADS2, and SLC27A4 genes. Three expression quantitative trail loci (eQTLs) corresponding to ACSM5, FABP4, and FADS2 were classifed as cis-acting eQTLs, whereas the remaining 16 eQTLs have trans-regulatory efects. Remarkably, a SNP in the ACSM5 promoter region and a SNP in the 3′UTR region of FABP4 were the most associated polymorphisms with the ACSM5 and FABP4 expression levels, respectively. Moreover, relevant lipid-related genes mapped in the trans-eQTLs regions associated with the ACSM5, FABP4, FADS2, and SLC27A4 genes. Interestingly, a trans-eQTL hotspot on SSC13 regulating the gene expression of ELOVL6, ELOLV5, and SCD, three important genes implicated in the elongation and desaturation of fatty acids, was identifed. These fndings provide new data to further understand the functional regulatory mechanisms implicated in the variation of fatty acid composition in pigs.
639 - Caça. Pesca. Piscicultura
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Revilla, Manuel, Anna Puig-Oliveras, Daniel Crespo-Piazuelo, Lourdes Criado-Mesas, Anna Castelló, Ana I. Fernández, Maria Ballester, and Josep M. Folch. 2018. "Expression Analysis Of Candidate Genes For Fatty Acid Composition In Adipose Tissue And Identification Of Regulatory Regions". Scientific Reports 8 (1). Springer Nature. doi:10.1038/s41598-018-20473-3.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-56369-C2-2-R/ES/VALIDACION GENETICA Y FUNCIONAL DE QTLS, GENES Y REDES GENICAS Y ESTUDIO DEL EFECTO DEL MICROBIOMA EN EL CRECIMIENTO, DEPOSICION DE GRASA Y CALIDAD DE LA CARNE EN PORCINO S2/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-82641-R/ES/GENOMICA FUNCIONAL, BIOLOGIA DE SISTEMAS Y MICROBIOMICA APLICADAS A LA IDENTIFICACION DE REGULADORES GENETICOS DEL CRECIMIENTO, ENGRASAMIENTO Y CALIDAD DE LA CARNE EN PORCINO/
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC‑2013‑12573/ES/ /
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
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