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dc.contributor.authorCasas, Ana M.
dc.contributor.authorContreras-Moreira, Bruno
dc.contributor.authorCantalapiedra, Carlos P.
dc.contributor.authorSakuma, Shun
dc.contributor.authorGracia, María Pilar
dc.contributor.authorMoralejo, Marian
dc.contributor.authorMolina-Cano, José Luis
dc.contributor.authorKomatsuda, Takao
dc.contributor.authorIgartua, Ernesto
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2019-06-04T08:40:59Z
dc.date.available2019-06-04T08:40:59Z
dc.date.issued2018-04-12
dc.identifier.citationCasas, Ana M., Bruno Contreras-Moreira, Carlos P. Cantalapiedra, Shun Sakuma, María Pilar Gracia, Marian Moralejo, José Luis Molina-Cano, Takao Komatsuda, and Ernesto Igartua. 2018. "Resequencing Thevrs1 Gene In Spanish Barley Landraces Revealed Reversion Of Six-Rowed To Two-Rowed Spike". Molecular Breeding 38 (5). Springer Nature. doi:10.1007/s11032-018-0816-z.ca
dc.identifier.issn1380-3743ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/412
dc.description.abstractSix-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists.ca
dc.format.extent25ca
dc.language.isoengca
dc.publisherSpringerca
dc.relation.ispartofMolecular Breedingca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleResequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spikeca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.terms12 mesosca
dc.relation.projectIDMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-21929/ES/DESCUBRIMIENTO DE NUEVA VARIABILIDAD PARA LA MEJORA DE LA CEBADA EN ESPAÑA/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48756-R/ES/DESCUBRIMIENTO Y UTILIZACION DE LA VARIABILIDAD GENETICA QUE DETERMINA LA ADAPTACION DE LA CEBADA MEDIANTE HERRAMIENTAS GENETICAS, GENOMICAS/ca
dc.relation.projectIDINIA/Programa Nacional de Proyectos de Investigación Fundamental/RFP2012-00015-00-00/ES/Mantenimiento de las colecciones de germoplasma en semilla de la Estación Experimental de Aula Dei (CSIC)/ca
dc.relation.projectIDINIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00033-C03-02/ES/Obtención de variedades de cebada de calidad con amplia adaptación en España/ca
dc.relation.projectIDMICINN/Programa Nacional de Internacionalización de la I+D/EUI2009-04075/ES/EXPLOITING GENETIC VARIATION FOR RESISTANCE TO IMPORTANT PATHOGENS IN BARLEY/ca
dc.relation.projectIDMICINN/Programa Nacional de Formación de Recursos Humanos/BES-2011-045905/ES/ /ca
dc.subject.udc633ca
dc.identifier.doihttps://doi.org/10.1007/s11032-018-0816-zca
dc.contributor.groupCultius Extensius Sosteniblesca


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