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dc.contributor.authorGonzález-Prendes, Rayner
dc.contributor.authorQuintanilla, Raquel
dc.contributor.authorMármol-Sánchez, Emilio
dc.contributor.authorPena, Ramona N.
dc.contributor.authorBallester, Maria
dc.contributor.authorCardoso, Tainã Figueiredo
dc.contributor.authorManunza, Arianna
dc.contributor.authorCasellas, Joaquim
dc.contributor.authorCánovas, Ángela
dc.contributor.authorDíaz, Isabel
dc.contributor.authorNoguera, José Luis
dc.contributor.authorCastelló, Anna
dc.contributor.authorMercadé, Anna
dc.contributor.authorAmills, Marcel
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-06-13T11:11:55Z
dc.date.available2019-06-13T11:11:55Z
dc.date.issued2019-03-04
dc.identifier.citationGonzález-Prendes, Rayner, Raquel Quintanilla, Emilio Mármol-Sánchez, Ramona N. Pena, Maria Ballester, Tainã Figueiredo Cardoso, and Arianna Manunza et al. 2019. "Comparing The Mrna Expression Profile And The Genetic Determinism Of Intramuscular Fat Traits In The Porcine Gluteus Medius And Longissimus Dorsi Muscles". BMC Genomics 20 (1). Springer Nature. doi:10.1186/s12864-019-5557-9.ca
dc.identifier.issn1471-2164ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/433
dc.description.abstractBackground Intramuscular fat (IMF) content and composition have a strong impact on the nutritional and organoleptic properties of porcine meat. The goal of the current work was to compare the patterns of gene expression and the genetic determinism of IMF traits in the porcine gluteus medius (GM) and longissimus dorsi (LD) muscles. Results A comparative analysis of the mRNA expression profiles of the pig GM and LD muscles in 16 Duroc pigs with available microarray mRNA expression measurements revealed the existence of 106 differentially expressed probes (fold-change > 1.5 and q-value < 0.05). Amongst the genes displaying the most significant differential expression, several loci belonging to the Hox transcription factor family were either upregulated (HOXA9, HOXA10, HOXB6, HOXB7 and TBX1) or downregulated (ARX) in the GM muscle. Differences in the expression of genes with key roles in carbohydrate and lipid metabolism (e.g. FABP3, ORMDL1 and SLC37A1) were also detected. By performing a GWAS for IMF content and composition traits recorded in the LD and GM muscles of 350 Duroc pigs, we identified the existence of one region on SSC14 (110–114 Mb) displaying significant associations with C18:0, C18:1(n-7), saturated and unsaturated fatty acid contents in both GM and LD muscles. Moreover, we detected several genome-wide significant associations that were not consistently found in both muscles. Further studies should be performed to confirm whether these associations are muscle-specific. Finally, the performance of an eQTL scan for 74 genes, located within GM QTL regions and with available microarray measurements of gene expression, made possible to identify 14 cis-eQTL regulating the expression of 14 loci, and six of them were confirmed by RNA-Seq. Conclusions We have detected significant differences in the mRNA expression patterns of the porcine LD and GM muscles, evidencing that the transcriptomic profile of the skeletal muscle tissue is affected by anatomical, metabolic and functional factors. A highly significant association with IMF composition on SSC14 was replicated in both muscles, highlighting the existence of a common genetic determinism, but we also observed the existence of a few associations whose magnitude and significance varied between LD and GM muscles.ca
dc.format.extent18ca
dc.language.isoengca
dc.publisherBMCca
dc.relation.ispartofBMC Genomicsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleComparing the mRNA expression profile and the genetic determinism of intramuscular fat traits in the porcine gluteus medius and longissimus dorsi musclesca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/ PROGRAMA ESTATAL DE INVESTIGACIÓN, DESARROLLO E INNOVACIÓN ORIENTADA A LOS RETOS DE LA SOCIEDAD/AGL2013-48742-C2-1-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/ca
dc.relation.projectIDMINECO/ PROGRAMA ESTATAL DE INVESTIGACIÓN, DESARROLLO E INNOVACIÓN ORIENTADA A LOS RETOS DE LA SOCIEDAD/AGL2013-48742-C2-2-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/ca
dc.relation.projectIDMINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /ca
dc.relation.projectIDMINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/RYC-2013-12573/ES/ /ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1186/s12864-019-5557-9ca
dc.contributor.groupGenètica i Millora Animalca


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