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Detection of isothermally amplified ostreid herpesvirus 1 DNA in Pacific oyster (Crassostrea gigas) using a miniaturised electrochemical biosensor
dc.contributor.author | Toldrà, Anna | |
dc.contributor.author | Furones, M. Dolors | |
dc.contributor.author | O'Sullivan, Ciara K. | |
dc.contributor.author | Campàs, Mònica | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2019-09-24T07:08:03Z | |
dc.date.available | 2022-03-24T12:00:23Z | |
dc.date.issued | 2019-09-02 | |
dc.identifier.citation | Toldrà, Anna, M. Dolors Furones, Ciara K. O'Sullivan, and Mònica Campàs. 2020. "Detection Of Isothermally Amplified Ostreid Herpesvirus 1 DNA In Pacific Oyster (Crassostrea Gigas) Using A Miniaturised Electrochemical Biosensor". Talanta 207: 120308. Elsevier BV. doi:10.1016/j.talanta.2019.120308. | ca |
dc.identifier.issn | 0039-9140 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/493 | |
dc.description.abstract | Given the threat that ostreid herpesvirus 1 (OsHV-1) poses to shellfish aquaculture, the need for rapid, user-friendly and cost-effective methods to detect this marine pathogen and minimise its impact is evident. In this work, an electrochemical biosensor for the detection of OsHV-1 based on isothermal recombinase polymerase amplification (RPA) was developed. The system was first tested and optimised on maleimide microtitre plates as a proof-of-concept, before being implemented on miniaturised gold electrodes. Amperometric detection of the isothermally amplified product was achieved through a sandwich hybridisation assay with an immobilised thiolated capture probe and a horseradish peroxidase (HRP)-labelled reporter probe. Calibration curves were constructed using PCR-amplified OsHV-1 DNA, achieving a limit of detection of 207 OsHV-1 target copies. The biosensor was applied to the analysis of 16 oyster samples from an infectivity experiment, and results were compared with those obtained by qPCR analysis, showing a strong degree of correlation (r = 0.988). The simplicity, rapidity, cost-effectiveness and potential for in-situ testing with the developed biosensor provide a valuable tool for the detection of OsHV-1 in aquaculture facilities, improving their management. | ca |
dc.format.extent | 24 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Talanta | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Detection of isothermally amplified ostreid herpesvirus 1 DNA in Pacific oyster (Crassostrea gigas) using a miniaturised electrochemical biosensor | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | EC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI | ca |
dc.subject.udc | 639 | ca |
dc.identifier.doi | https://doi.org/10.1016/j.talanta.2019.120308 | ca |
dc.contributor.group | Aigües Marines i Continentals | ca |
dc.contributor.group | Aqüicultura | ca |
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