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dc.contributor.authorToldrà, Anna
dc.contributor.authorFurones, M. Dolors
dc.contributor.authorO'Sullivan, Ciara K.
dc.contributor.authorCampàs, Mònica
dc.contributor.otherProducció Animalca
dc.identifier.citationToldrà, Anna, M. Dolors Furones, Ciara K. O'Sullivan, and Mònica Campàs. 2020. "Detection Of Isothermally Amplified Ostreid Herpesvirus 1 DNA In Pacific Oyster (Crassostrea Gigas) Using A Miniaturised Electrochemical Biosensor". Talanta 207: 120308. Elsevier BV. doi:10.1016/
dc.description.abstractGiven the threat that ostreid herpesvirus 1 (OsHV-1) poses to shellfish aquaculture, the need for rapid, user-friendly and cost-effective methods to detect this marine pathogen and minimise its impact is evident. In this work, an electrochemical biosensor for the detection of OsHV-1 based on isothermal recombinase polymerase amplification (RPA) was developed. The system was first tested and optimised on maleimide microtitre plates as a proof-of-concept, before being implemented on miniaturised gold electrodes. Amperometric detection of the isothermally amplified product was achieved through a sandwich hybridisation assay with an immobilised thiolated capture probe and a horseradish peroxidase (HRP)-labelled reporter probe. Calibration curves were constructed using PCR-amplified OsHV-1 DNA, achieving a limit of detection of 207 OsHV-1 target copies. The biosensor was applied to the analysis of 16 oyster samples from an infectivity experiment, and results were compared with those obtained by qPCR analysis, showing a strong degree of correlation (r = 0.988). The simplicity, rapidity, cost-effectiveness and potential for in-situ testing with the developed biosensor provide a valuable tool for the detection of OsHV-1 in aquaculture facilities, improving their
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.titleDetection of isothermally amplified ostreid herpesvirus 1 DNA in Pacific oyster (Crassostrea gigas) using a miniaturised electrochemical biosensorca
dc.embargo.terms24 mesosca
dc.relation.projectIDEC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDIca
dc.contributor.groupAigües Marines i Continentalsca

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