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dc.contributor.authorMármol-Sánchez, Emilio
dc.contributor.authorRamayo-Caldas, Yuliaxis
dc.contributor.authorQuintanilla, Raquel
dc.contributor.authorCardoso, Tainã Figueiredo
dc.contributor.authorGonzález-Prendes, Rayner
dc.contributor.authorTibau, Joan
dc.contributor.authorAmills, Marcel
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-03-11T16:13:10Z
dc.date.available2020-03-11T16:13:10Z
dc.date.issued2020-01-17
dc.identifier.citationMármol-Sánchez, Emilio, Yuliaxis Ramayo-Caldas, Raquel Quintanilla, Tainã Figueiredo Cardoso, Rayner González-Prendes, Joan Tibau, and Marcel Amills. 2020. "Co-Expression Network Analysis Predicts A Key Role Of Micrornas In The Adaptation Of The Porcine Skeletal Muscle To Nutrient Supply". Journal Of Animal Science And Biotechnology 11 (1). Springer Science and Business Media LLC. doi:10.1186/s40104-019-0412-z.ca
dc.identifier.issn1674-9782ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/639
dc.description.abstractBackground The role of non-coding RNAs in the porcine muscle metabolism is poorly understood, with few studies investigating their expression patterns in response to nutrient supply. Therefore, we aimed to investigate the changes in microRNAs (miRNAs), long intergenic non-coding RNAs (lincRNAs) and mRNAs muscle expression before and after food intake. Results We measured the miRNA, lincRNA and mRNA expression levels in the gluteus medius muscle of 12 gilts in a fasting condition (AL-T0) and 24 gilts fed ad libitum during either 5 h. (AL-T1, N = 12) or 7 h. (AL-T2, N = 12) prior to slaughter. The small RNA fraction was extracted from muscle samples retrieved from the 36 gilts and sequenced, whereas lincRNA and mRNA expression data were already available. In terms of mean and variance, the expression profiles of miRNAs and lincRNAs in the porcine muscle were quite different than those of mRNAs. Food intake induced the differential expression of 149 (AL-T0/AL-T1) and 435 (AL-T0/AL-T2) mRNAs, 6 (AL-T0/AL-T1) and 28 (AL-T0/AL-T2) miRNAs and none lincRNAs, while the number of differentially dispersed genes was much lower. Among the set of differentially expressed miRNAs, we identified ssc-miR-148a-3p, ssc-miR-22-3p and ssc-miR-1, which play key roles in the regulation of glucose and lipid metabolism. Besides, co-expression network analyses revealed several miRNAs that putatively interact with mRNAs playing key metabolic roles and that also showed differential expression before and after feeding. One case example was represented by seven miRNAs (ssc-miR-148a-3p, ssc-miR-151-3p, ssc-miR-30a-3p, ssc-miR-30e-3p, ssc-miR-421-5p, ssc-miR-493-5p and ssc-miR-503) which putatively interact with the PDK4 mRNA, one of the master regulators of glucose utilization and fatty acid oxidation. Conclusions As a whole, our results evidence that microRNAs are likely to play an important role in the porcine skeletal muscle metabolic adaptation to nutrient availability.ca
dc.format.extent21ca
dc.language.isoengca
dc.publisherBMCca
dc.relation.ispartofJournal of Animal Science and Biotechnologyca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleCo-expression network analysis predicts a key role of microRNAs in the adaptation of the porcine skeletal muscle to nutrient supplyca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDEC/H2020/665919/EU/Opening Sphere UAB-CEI to PostDoctoral Fellows/P-SPHEREca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48742-C2-1-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48742-C2-2-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/ca
dc.relation.projectIDMINECO/ /SEV-2015-0533/ES/ /ca
dc.subject.udc59ca
dc.subject.udc619ca
dc.subject.udc636ca
dc.identifier.doihttps://doi.org/10.1186/s40104-019-0412-zca
dc.contributor.groupGenètica i Millora Animalca


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