Toxicity characterisation of Gambierdiscus species from the Canary Islands
Rossignoli, Araceli E.
Díaz, Patricio A.
In the last decade, several outbreaks of ciguatera fish poisoning (CFP) have been reported in the Canary Islands (central northeast Atlantic Ocean), confirming ciguatera as an emerging alimentary risk in this region. Five Gambierdiscus species, G. australes, G. excentricus, G. silvae, G. carolinianus and G. caribaeus, have been detected in macrophytes from this area and are known to produce the ciguatoxins (CTXs) that cause CFP. A characterization of the toxicity of these species is the first step in identifying locations in the Canary Islands at risk of CFP. Therefore, in this study the toxicity of 63 strains of these five Gambierdiscus species were analysed using the erythrocyte lysis assay to evaluate their maitotoxin (MTX) content. In addition, 20 of the strains were also analysed in a neuroblastoma Neuro-2a (N2a) cytotoxicity assay to determine their CTX-like toxicity. The results allowed the different species to be grouped according to their ratios of CTX-like and MTX-like toxicity. MTX-like toxicity was especially high in G. excentricus and G. australes but much lower in the other species and lowest in G. silvae. CTX-like toxicity was highest in G. excentricus, which produced the toxin in amounts ranging between 128.2 ± 25.68 and 510.6 ± 134.2 fg CTX1B equivalents (eq) cell−1 (mean ± SD). In the other species, CTX concentrations were as follows: G. carolinianus (100.84 ± 18.05 fg CTX1B eq cell−1), G. australes (31.1 ± 0.56 to 107.16 ± 21.88 fg CTX1B eq cell−1), G. silvae (12.19 ± 0.62 to 76.79 ± 4.97 fg CTX1B eq cell−1) and G. caribaeus (<LOD to 90.37 ± 15.89 fg CTX1B eq cell−1). Unlike the similar CTX-like toxicity of G. australes and G. silvae strains from different locations, G. excentricus and G. caribaeus differed considerably according to the origin of the strain. These differences emphasise the importance of species identification to assess the regional risk of CFP.
574 - General ecology and biodiversity
Is part of
Rossignoli, Araceli E., Angels Tudó, Isabel Bravo, Patricio A. Díaz, Jorge Diogène, and Pilar Riobó. 2020. "Toxicity Characterisation Of Gambierdiscus Species From The Canary Islands". Toxins 12 (2): 134. MDPI AG. doi:10.3390/toxins12020134.
Grant agreement number
EFSA/ /GP-EFSA-AFSCO-2015-03/EU/Risk Characterization of Ciguatera Food Poisoning in Europe/EUROCIGUA
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/CGL2013-40671-R/ES/ESTRATEGIAS ADAPTATIVAS DE MICROALGAS NOCIVAS: UNA APROXIMACION INNOVADORA APLICADA AL ESTUDIO DE LA CIGUATERA EN CANARIAS ANTE EL CAMBIO CLIMATICO/CICAN
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/CTM2017-86066-R/ES/DETECCIÓN INNOVADORA DE PROLIFERACIONES ALGALES TÓXICAS: UNA NECESIDAD FRENTE AL CALENTAMIENTO GLOBAL/DIANAS
Aigües Marines i Continentals
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- ARTICLES CIENTÍFICS 
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