A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish
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Author
Rambla-Alegre, Maria
Campbell, Katrina
Gerssen, Arjen
Publication date
2020-04-05ISSN
0278-6915
Abstract
Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
16
Publisher
Elsevier
Is part of
Food and Chemical Toxicology
Citation
Campàs, Mònica, Jaume Reverté, Maria Rambla-Alegre, Katrina Campbell, Arjen Gerssen, and Jorge Diogène. 2020. "A Fast Magnetic Bead-Based Colorimetric Immunoassay For The Detection Of Tetrodotoxins In Shellfish". Food And Chemical Toxicology 140: 111315. Elsevier BV. doi:10.1016/j.fct.2020.111315.
Grant agreement number
EC/H2020/773400/EU/Nutritious, safe and sustainable seafood for consumers of tomorrow/SEAFOODTOMORROW
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2850]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/