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dc.contributor.authorZhou, Xue
dc.contributor.authorZhang, Xiu-Xiang
dc.contributor.authorMahmmod, Yasser S.
dc.contributor.authorHernandez, Jorge A.
dc.contributor.authorLi, Gui-Feng
dc.contributor.authorHuang, Wan-Yi
dc.contributor.authorWang, Ya-Pei
dc.contributor.authorZheng, Yu-Xiang
dc.contributor.authorLi, Xiu-Ming
dc.contributor.authorYuan, Zi-Guo
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-04-27T06:33:34Z
dc.date.available2020-04-27T06:33:34Z
dc.date.issued2020-02-19
dc.identifier.citationZhou, Xue, Xiu-Xiang Zhang, Yasser S. Mahmmod, Jorge A. Hernandez, Gui-Feng Li, Wan-Yi Huang, Ya-Pei Wang, Yu-Xiang Zheng, Xiu-Ming Li, and Zi-Guo Yuan. 2020. "A Transcriptome Analysis: Various Reasons Of Adverse Pregnancy Outcomes Caused By Acute Toxoplasma Gondii Infection". Frontiers In Physiology 11. Frontiers Media SA. doi:10.3389/fphys.2020.00115.ca
dc.identifier.issn1664-042Xca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/745
dc.description.abstractBackground: Toxoplasma gondii (T. gondii) is an obligate intracellular parasite, which can affect the pregnancy outcomes in infected females by damaging the uterus, and the intrauterine environment as well as and the hypothalamus resulting in hormonal imbalance. However, the molecular mechanisms underlying the parasite-induced poor pregnancy outcomes and the key genes regulating these mechanisms remain unclear. Therefore, this study aimed to analyze the gene expression in the mouse’s uterus following experimentally-induced acute infection with T. gondii RH strain. Three groups of female mice were intraperitoneally injected with tachyzoites as follow; 3 days before pregnancy (FBD6), after pregnancy (FAD6), and after implantation (FID8) as the experimental groups. Another corresponding three groups served as control, were injected with normal saline at the same time. Transcriptome analysis of the total RNA extracted from both infected and non-infected mouse uterus samples was performed using RNA sequencing (RNA-Seq). Results: The three experimental groups (FBD6, FAD6, and FID8) had a total of 4,561, 2,345, and 2,997 differentially expressed genes (DEGs) compared to the controls. The significantly upregulated and downregulated DEGs were 2,571 and 1,990 genes in FBD6, 1,042 and 1,303 genes in FAD6 and 1,162 and 1,835 genes in FID8 group, respectively. The analysis of GO annotation, and KEGG pathway showed that DEGs were mainly involved in anatomical structure development, transport, cell differentiation, embryo development, hormone biosynthetic process, signal transduction, immune system process, phagosome, pathways in cancer, and cytokine-cytokine receptor interaction pathways. Conclusion: T. gondii infection can induce global transcriptomic changes in the uterus that may cause pregnancy hypertension, destruct the intrauterine environment, and hinder the normal development of placenta and embryo. Our results may help to understand the molecular mechanisms of the acute T. gondii infection, which could promote the development of new therapeutics or prophylactics for toxoplasmosis in pregnancy.ca
dc.format.extent15ca
dc.language.isoengca
dc.publisherFrontiers Mediaca
dc.relation.ispartofFrontiers in Physiologyca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA transcriptome analysis: Various reasons of adverse pregnancy outcomes caused by acute Toxoplasma gondii Infectionca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3389/fphys.2020.00115ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/