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dc.contributor.authorBolte, Josef
dc.contributor.authorZhang, Yanchao
dc.contributor.authorWente, Nicole
dc.contributor.authorMahmmod, Yasser S.
dc.contributor.authorSvennesen, Line
dc.contributor.authorKrömker, Volker
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-05-05T12:10:54Z
dc.date.issued2020-02-20
dc.identifier.citationBolte, Josef, Yanchao Zhang, Nicole Wente, Yasser S. Mahmmod, Line Svennesen, and Volker Krömker. 2020. "Comparison Of Phenotypic And Genotypic Antimicrobial Resistance Patterns Associated With Staphylococcus Aureus Mastitis In German And Danish Dairy Cows". Journal Of Dairy Science 103 (4): 3554-3564. American Dairy Science Association. doi:10.3168/jds.2019-17765.ca
dc.identifier.issn0022-0302ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/778
dc.description.abstractStaphylococcus aureus is one of the most common pathogens associated with bovine mastitis in Germany and Denmark. Successful therapy is strongly linked to the susceptibility of the pathogen to the administered antimicrobial. An increase in resistant pathogens in human and veterinary medicine has become a concern worldwide and hampers therapy due to reduced susceptibility. In the present study, susceptibility testing was performed for 85 and 93 S. aureus isolates originating from mastitis cases on 12 German and 8 Danish dairy farms, respectively. Phenotypic examination was performed by detection of minimal inhibitory concentration (MIC) values using the broth microdilution method, followed by genotypic investigations of the blaZ and mecA resistance genes via PCR. The tested antimicrobials were the most frequently used β-lactams in German and Danish dairy farms, including cefquinome, cefoperazone, cephapirin, penicillin, oxacillin, cloxacillin, amoxicillin-clavulanic acid, and cephalexin-kanamycin. Special attention was paid to varying therapy concepts because, in Germany, third- and fourth-generation cephalosporins have been predominantly used in mastitis therapy, whereas in Denmark, restrictive use of penicillin is followed by a general avoidance of cephalosporins. Differences in MIC values were mainly based on determined MIC90 values (MIC at which 90% of isolates are inhibited). In general, Danish S. aureus isolates were inhibited at comparatively lower MIC90 values than S. aureus isolated from German dairy farms for most β-lactams. No differences were observed regarding cefquinome, because both German and Danish isolates exhibited MIC50 and MIC90 values of 0.5 and 1 µg/mL, respectively. In contrast, the MIC90 for penicillin against German and Danish S. aureus were 0.5 and ≤0.06 µg/mL, respectively. Resistance genes (blaZ, mecA) were only detected in German S. aureus isolates on 3 dairy farms in Germany. A total of 5 isolates tested positive for both blaZ and mecA, whereas 1 isolate carried the blaZ resistance gene only. A direct correlation between frequently used antimicrobials and reduced susceptibility could not be determined based on results of the present study. In addition to further research to determine factors associated with resistance development, we emphasize the urgent need for internationally standardized clinical breakpoints to assess resistance situations more accurately.ca
dc.format.extent36ca
dc.language.isoengca
dc.publisherAmerican Dairy Science Associationca
dc.relation.ispartofJournal of Dairy Scienceca
dc.rights© 2020 American Dairy Science Association®ca
dc.titleComparison of phenotypic and genotypic antimicrobial resistance patterns associated with Staphylococcus aureus mastitis in German and Danish dairy cowsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/embargoedAccess
dc.date.embargoEnd2021-02-19T01:00:00Z
dc.embargo.terms12 mesosca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3168/jds.2019-17765ca
dc.contributor.groupSanitat Animalca


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