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dc.contributor.authorBirnberg, Lotty
dc.contributor.authorTemmam, Sarah
dc.contributor.authorAranda, Carles
dc.contributor.authorCorrea-Fiz, Florencia
dc.contributor.authorTalavera, Sandra
dc.contributor.authorBigot, Thomas
dc.contributor.authorEloit, Marc
dc.contributor.authorBusquets, Núria
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-05-18T09:23:24Z
dc.date.available2020-05-18T09:23:24Z
dc.date.issued2020-02-29
dc.identifier.citationBirnberg, Lotty, Sarah Temmam, Carles Aranda, Florencia Correa-Fiz, Sandra Talavera, Thomas Bigot, Marc Eloit, and Núria Busquets. 2020. "Viromics On Honey-Baited FTA Cards As A New Tool For The Detection Of Circulating Viruses In Mosquitoes". Viruses 12 (3): 274. MDPI AG. doi:10.3390/v12030274.ca
dc.identifier.issn1999-4915ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/795
dc.description.abstractWorldwide, emerging and re-emerging infectious diseases (EIDs) are a major burden on public and animal health. Arthropod vectors, with mosquitoes being the main contributors of global disease, transmit more than 70% of the recognized EIDs. To assess new alternatives for arthropod-borne viral diseases surveillance, and for the detection of new viruses, honey-baited Flinders Technology Associates (FTA) cards were used as sugar bait in mosquito traps during entomological surveys at the Llobregat River Delta (Catalonia, Spain). Next generation sequencing (NGS) metagenomics analysis was applied on honey-baited FTA cards, which had been exposed to field-captured mosquitoes to characterize their associated virome. Arthropod- and plant-infecting viruses governed the virome profile on FTA cards. Twelve near-complete viral genomes were successfully obtained, suggesting good quality preservation of viral RNAs. Mosquito pools linked to the FTA cards were screened for the detection of mosquito-associated viruses by specific RT-PCRs to confirm the presence of these viruses. The circulation of viruses related to Alphamesonivirus, Quaranjavirus and unclassified Bunyavirales was detected in mosquitoes, and phylogenetic analyses revealed their similarities to viruses previously reported in other continents. To the best our knowledge, our findings constitute the first distribution record of these viruses in European mosquitoes and the first hint of insect-specific viruses in mosquitoes’ saliva in field conditions, demonstrating the feasibility of this approach to monitor the transmissible fraction of the mosquitoes’ virome. In conclusion, this pilot viromics study on honey-baited FTA cards was shown to be a valid approach for the detection of viruses circulating in mosquitoes, thereby setting up an alternative tool for arbovirus surveillance and control programs.ca
dc.format.extent19ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofVirusesca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleViromics on honey-baited FTA cards as a new tool for the detection of circulating viruses in mosquitoesca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDEC/H2020/731060/EU/Research Infrastructures for the control of vector-borne diseases/INFRAVEC2ca
dc.relation.projectIDEC/FP7/613996/EU/Emerging, Viral Vector-Borne Diseases/VMERGEca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3390/v12030274ca
dc.contributor.groupSanitat Animalca


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