Fine mapping of the peach pollen sterility gene (Ps/ps) and detection of markers for marker-assisted selection
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Author
Eduardo, I.
de Tomás, C.
Alexiou, K. G.
Giovannini, D.
Pietrella, M.
Carpenedo, S.
Bassols Raseira, M. C.
Batlle, I.
Cantín, M. C.
Aranzana, M. J.
Arús, P.
Publication date
2020-06-10ISSN
1380-3743
Abstract
In peach, pollen sterility, expressed as absence of pollen in the anthers, segregates as an undesired trait in breeding programs. Pollen fertility screening in progenies is not a common practice mainly because it does not affect fruit set since cross-pollination is frequent. It is also a time-consuming activity that coincides with the busy pollination season. Segregation for this trait could be avoided by using molecular markers to identify appropriate parents or male sterile plants for early culling in progenies expected to segregate, thus increasing breeding efficiency. In peach, pollen sterility is determined by a recessive allele in homozygosis of the major gene, Ps/ps, located on chromosome 6. In this work, using a conventional mapping approach combined with bulked segregant analysis using resequencing data, we fine mapped Ps to a region of almost 160 kb and developed molecular markers for marker-assisted breeding. These markers were validated in plant materials from three peach breeding programs, including progenies, advanced selections, and cultivars, allowing us to determine that the frequency of the ps allele is high (0.23) and also to infer the genotypes of a large collection of cultivars and advanced breeding lines.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
663/664 - Food and nutrition. Enology. Oils. Fat
Pages
23
Publisher
Springer
Is part of
Molecular Breeding
Citation
Eduardo, I, C de Tomás, KG Alexiou, D Giovannini, M Pietrella, S Carpenedo, and MC Bassols Raseira et al. 2020. "Fine Mapping Of The Peach Pollen Sterility Gene (Ps/Ps) And Detection Of Markers For Marker-Assisted Selection". Molecular Breeding 40 (6). doi:10.1007/s11032-020-01139-3.
Grant agreement number
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-68329-R/ES/DENTIFICACION Y CARACTERIZACION DE GENES IMPLICADOS EN LA FORMA Y JUGOSIDAD DEL FRUTO EN MELOCOTONERO Y ALMENDRO/ALMELO
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2820]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/