dc.contributor.author | Criado-Mesas, Lourdes | |
dc.contributor.author | Ballester, Maria | |
dc.contributor.author | Crespo-Piazuelo, Daniel | |
dc.contributor.author | Castelló, Anna | |
dc.contributor.author | Fernández, Ana I. | |
dc.contributor.author | Folch, Josep M. | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2020-10-14T12:43:36Z | |
dc.date.available | 2020-10-14T12:43:36Z | |
dc.date.issued | 2020-06-17 | |
dc.identifier.citation | Criado-Mesas, Lourdes, Maria Ballester, Daniel Crespo-Piazuelo, Anna Castelló, Ana I. Fernández, and Josep M. Folch. 2020. "Identification Of Eqtls Associated With Lipid Metabolism In Longissimus Dorsi Muscle Of Pigs With Different Genetic Backgrounds". Scientific Reports 10 (1). doi:10.1038/s41598-020-67015-4. | ca |
dc.identifier.issn | 2045-2322 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/920 | |
dc.description.abstract | Intramuscular fat content and its fatty acid composition affect porcine meat quality and its nutritional value. The present work aimed to identify genomic variants regulating the expression in the porcine muscle (Longissimus dorsi) of 45 candidate genes for lipid metabolism and fatty acid composition in three experimental backcrosses based on the Iberian breed. Expression genome-wide association studies (eGWAS) were performed between the muscle gene expression values, measured by real-time quantitative PCR, and the genotypes of 38,426 SNPs distributed along all chromosomes. The eGWAS identified 186 eSNPs located in ten Sus scrofa regions and associated with the expression of ACSM5, ACSS2, ATF3, DGAT2, FOS and IGF2 (FDR < 0.05) genes. Two expression quantitative trait loci (eQTLs) for IGF2 and ACSM5 were classified as cis-acting eQTLs, suggesting a mutation in the same gene affecting its expression. Conversely, ten eQTLs showed trans-regulatory effects on gene expression. When the eGWAS was performed for each backcross independently, only three common trans-eQTL regions were observed, indicating different regulatory mechanisms or allelic frequencies among the breeds. In addition, hotspot regions regulating the expression of several genes were detected. Our results provide new data to better understand the functional regulatory mechanisms of lipid metabolism genes in muscle. | ca |
dc.format.extent | 13 | ca |
dc.language.iso | eng | ca |
dc.publisher | Nature Research | ca |
dc.relation.ispartof | Scientific Reports | ca |
dc.rights | Attribution 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Identification of eQTLs associated with lipid metabolism in Longissimus dorsi muscle of pigs with different genetic backgrounds | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MINECO-FEDER/Programa estatal de I+D+I orientada a los retos de la sociedad/AGL2014-56369-C2-2-R/ES//Validación genética y funcional de QTLS, genes y redes génicas y estudio del efecto del microbioma sobre crecimiento, deposición de grasa y calidad de carne en porcino. S2/ | ca |
dc.relation.projectID | MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-82641-R/ES/Genómica funcional, biología de sistemas y microbiomica aplicadas a la identificación de reguladores genéticos del crecimiento, engrasamiento y calidad de la carne en porcino/ | ca |
dc.relation.projectID | MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC‑2013‑12573/ES/ / | ca |
dc.relation.projectID | MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ / | ca |
dc.subject.udc | 57 | ca |
dc.subject.udc | 573 | ca |
dc.subject.udc | 575 | ca |
dc.subject.udc | 59 | ca |
dc.subject.udc | 619 | ca |
dc.subject.udc | 636 | ca |
dc.identifier.doi | https://doi.org/10.1038/s41598-020-67015-4 | ca |
dc.contributor.group | Genètica i Millora Animal | ca |