Show simple item record

dc.contributor.authorOlsvik, Pål A.
dc.contributor.authorRoderick, Nigel Finn
dc.contributor.authorRemø, Sofie C.
dc.contributor.authorFjelldal, Per G.
dc.contributor.authorChauvigné, François
dc.contributor.authorGlover, Kevin A.
dc.contributor.authorHansen, Tom
dc.contributor.authorWaagbø, Rune
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-10-21T18:25:18Z
dc.date.available2020-10-21T18:25:18Z
dc.date.issued2020-07-28
dc.identifier.citationOlsvik, Pål A., Roderick Nigel Finn, Sofie C. Remø, Per G. Fjelldal, François Chauvigné, Kevin A. Glover, Tom Hansen, and Rune Waagbø. 2020. "A Transcriptomic Analysis Of Diploid And Triploid Atlantic Salmon Lenses With And Without Cataracts". Experimental Eye Research 199: 108150. doi:10.1016/j.exer.2020.108150.ca
dc.identifier.issn0014-4835ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/937
dc.description.abstractTo avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology compared to diploid fish, with increased prevalence of vertebral deformities and ocular cataracts as two of the most severe disorders. Here, we investigated the mechanisms behind the higher prevalence of cataracts in triploid salmon, by comparing the transcriptional patterns in lenses of diploid and triploid Atlantic salmon, with and without cataracts. We assembled and characterized the Atlantic salmon lens transcriptome and used RNA-seq to search for the molecular basis for cataract development in triploid fish. Transcriptional screening showed only modest differences in lens mRNA levels in diploid and triploid fish, with few uniquely expressed genes. In total, there were 165 differentially expressed genes (DEGs) between the cataractous diploid and triploid lens. Of these, most were expressed at lower levels in triploid fish. Differential expression was observed for genes encoding proteins with known function in the retina (phototransduction) and proteins associated with repair and compensation mechanisms. The results suggest a higher susceptibility to oxidative stress in triploid lenses, and that mechanisms connected to the ability to handle damaged proteins are differentially affected in cataractous lenses from diploid and triploid salmon.ca
dc.format.extent14ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofExperimental Eye Researchca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataractsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc639ca
dc.identifier.doihttps://doi.org/10.1016/j.exer.2020.108150ca
dc.contributor.groupAqüiculturaca


Files in this item

 

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/