Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment
Ur Rahmann, Faiz
Andree, Karl B.
Figueras, Maria J.
Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p < 0.05) among broths were calculated by ANOVA. The results indicated that shellfish-associated Arcobacter-like species growth was significantly higher in Arcobacter broth + 50% ASW and the same media supplemented with lyophilized oysters. This is the first study to have used fresh or lyophilized oyster flesh in the enrichment broth for isolation of shellfish-associated Arcobacter-like spp.
639 - Caça. Pesca. Piscicultura
Springer Nature Limited
Is part of
Rahman, Faiz Ur, Karl B. Andree, Nuria Salas-Massó, Margarita Fernandez-Tejedor, Anna Sanjuan, Maria J. Figueras, and M. Dolors Furones. 2020. "Improved Culture Enrichment Broth For Isolation Of Arcobacter-Like Species From The Marine Environment". Scientific Reports 10 (1). doi:10.1038/s41598-020-71442-8.
Grant agreement number
EC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/E-RTA2015-00004-00-00/ES/Gestión sanitaria integrada de las enfermedades emergentes de bivalvos con interés comercial en el Mediterráneo español/EMERGER
Aigües Marines i Continentals
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