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dc.contributor.authorHaverkamp, Ann-Kathrin
dc.contributor.authorLehmbecker, Annika
dc.contributor.authorSpitzbarth, Ingo
dc.contributor.authorWidagdo, Widagdo
dc.contributor.authorHaagmans, Bart L.
dc.contributor.authorSegalés, Joaquim
dc.contributor.authorVergara-Alert, Julia
dc.contributor.authorBensaid, Albert
dc.contributor.authorvan den Brand, Judith M. A.
dc.contributor.authorOsterhaus, Albert D. M. E.
dc.contributor.authorBaumgärtner, Wolfgang
dc.contributor.otherProducció Animalca
dc.date.accessioned2018-12-03T13:04:17Z
dc.date.available2018-12-03T13:04:17Z
dc.date.issued2018-06-27
dc.identifier.citationHaverkamp, Ann-Kathrin, Annika Lehmbecker, Ingo Spitzbarth, Widagdo Widagdo, Bart L. Haagmans, Joaquim Segalés, and Julia Vergara-Alert et al. 2018. "Experimental Infection Of Dromedaries With Middle East Respiratory Syndrome-Coronavirus Is Accompanied By Massive Ciliary Loss And Depletion Of The Cell Surface Receptor Dipeptidyl Peptidase 4". Scientific Reports 8 (1). Springer Nature. doi:10.1038/s41598-018-28109-2.ca
dc.identifier.issn2045-2322ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/96
dc.description.abstractMiddle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one-third of human patients. Recent data indicate that dromedaries represent an important source of infection, although information regarding viral cell tropism and pathogenesis is sparse. In the current study, tissues of eight dromedaries receiving inoculation of MERS-Coronavirus (MERS-CoV) after recombinant Modified-Vaccinia-Virus-Ankara (MVA-S)-vaccination (n = 4), MVA-vaccination (mock vaccination, n = 2) and PBS application (mock vaccination, n = 2), respectively, were investigated. Tissues were analyzed by histology, immunohistochemistry, immunofluorescence, and scanning electron microscopy. MERS-CoV infection in mock-vaccinated dromedaries revealed high numbers of MERS-CoV-nucleocapsid positive cells, T cells, and macrophages within nasal turbinates and trachea at day four post infection. Double immunolabeling demonstrated cytokeratin (CK) 18 expressing epithelial cells to be the prevailing target cell of MERS-CoV, while CK5/6 and CK14 expressing cells did not co-localize with virus. In addition, virus was occasionally detected in macrophages. The acute disease was further accompanied by ciliary loss along with a lack of dipeptidyl peptidase 4 (DPP4), known to mediate virus entry. DPP4 was mainly expressed by human lymphocytes and dromedary monocytes, but overall the expression level was lower in dromedaries. The present study underlines significant species-specific manifestations of MERS and highlights ciliary loss as an important finding in dromedaries. The obtained results promote a better understanding of coronavirus infections, which pose major health challenges.ca
dc.format.extent15ca
dc.language.isoengca
dc.publisherNature Researchca
dc.relation.ispartofScientific Reportsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleExperimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4ca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDEC/PF7/115760/EU/Zoonotic Anticipation and Preparedness Initiative/ZAPIca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1038/s41598-018-28109-2ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/
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