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dc.contributor.authorMarimon, Neus
dc.contributor.authorLuque, Jordi
dc.contributor.authorArús, Pere
dc.contributor.authorEduardo, Iban
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2020-11-06T12:54:28Z
dc.date.available2020-11-06T12:54:28Z
dc.date.issued2020-11-01
dc.identifier.citationMarimon, Neus, Jordi Luque, Pere Arús, and Iban Eduardo. 2020. "Fine Mapping And Identification Of Candidate Genes For The Peach Powdery Mildew Resistance Gene Vr3". Horticulture Research 7 (1). doi:10.1038/s41438-020-00396-9.ca
dc.identifier.issn2052-7276ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/975
dc.description.abstractPowdery mildew is one of the major diseases of peach (Prunus persica), caused by the ascomycete Podosphaera pannosa. Currently, it is controlled through calendar-based fungicide treatments starting at petal fall, but an alternative is to develop resistant peach varieties. Previous studies mapped a resistance gene (Vr3) in interspecific populations between almond (‘Texas’) and peach (‘Earlygold’). To obtain molecular markers highly linked to Vr3 and to reduce the number of candidate genes, we fine-mapped Vr3 to a genomic region of 270 kb with 27 annotated genes. To find evidence supporting one of these positional candidate genes as being responsible of Vr3, we analyzed the polymorphisms of the resequences of both parents and used near-isogenic lines (NILs) for expression analysis of the positional candidate genes in symptomatic or asymptomatic leaves. Genes differentially expressed between resistant and susceptible individuals were annotated as a Disease Resistance Protein RGA2 (Prupe2G111700) or an Eceriferum 1 protein involved in epicuticular wax biosynthesis (Prupe2G112800). Only Prupe2G111700 contained a variant predicted to have a disruptive effect on the encoded protein, and was overexpressed in both heterozygous and homozygous individuals containing the Vr3 almond allele, compared with susceptible individuals. This information was also useful to identify and validate molecular markers tightly linked and flanking Vr3. In addition, the NILs used in this work will facilitate the introgression of this gene into peach elite materials, alone or pyramided with other known resistance genes such as peach powdery mildew resistance gene Vr2.ca
dc.format.extent9ca
dc.language.isoengca
dc.publisherSpringer Natureca
dc.relation.ispartofHorticulture Researchca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleFine mapping and identification of candidate genes for the peach powdery mildew resistance gene Vr3ca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDINIA-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RTA2013-00004-C03-01/ES/Estrategias de control de enfermedades fúngicas aéreas y de raíz en frutales mediterráneos, basadas en criterios epidemiológicos y en la utilización de micorrizas arbusculares/ca
dc.relation.projectIDINIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00050-00-00/ES/Nuevas estrategias de mejora del melocotonero basadas en marcadores moleculares: introgresión asistida por marcadores y resíntesis/ca
dc.relation.projectIDMINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /ca
dc.relation.projectIDMINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-68329-R/ES/Identificación y caracterización de genes implicados en la forma y jugosidad del fruto en melocotonero y almendro/ALMELOca
dc.subject.udc633ca
dc.identifier.doihttps://doi.org/10.1038/s41438-020-00396-9ca
dc.contributor.groupGenòmica i Biotecnologiaca
dc.contributor.groupProtecció Vegetal Sostenibleca


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