Use of anionic polymer-coated magnetic beads to pre-concentrate Ostreid Herpesvirus 1 from seawater: Application to a UV disinfection treatment
Visualitza/Obre
Autor/a
Toldrà, Anna
Andree, Karl B.
Lowenthal, Assaf
Rozenberg, Ytzhak
Furones, M. Dolors
Data de publicació
2021-02-02ISSN
0044-8486
Resum
Ostreid Herpesvirus 1 (OsHV-1) represents a serious threat to shellfish aquaculture worldwide. To minimise its impact, early warning systems able to detect the virus in seawater prior to infection of oysters are of utmost importance. However, monitoring OsHV-1 in seawater is challenging because of its presence at very low concentrations. Thus, a rapid and simple method to pre-concentrate the virus is needed to enable detection. Herein, magnetic beads (MBs) coated with an anionic polymer were used to pre-concentrate OsHV-1 from biological matrices including oyster homogenate and seawater samples. Following virus capture, OsHV-1 DNA detection was performed by quantitative PCR (qPCR). The MB-based approach combined with qPCR attained a limit of detection (LOD) as low as 0.1 viral copy/μL, which was 100 times lower than that of the qPCR alone. This approach was applied to the analysis of OsHV-1 in seawater from an Enhanced Hydro-Optic UV (HOD-UV) disinfection experiment operating at a UV dose of 1360 J/m2 in an open flow system. Our approach enabled detection of the virus in non-treated seawater and not in UV-treated seawater, discrimination that was not possible using qPCR alone. Moreover, the strategy provided data on the pattern of kinetics of the release of the virus in seawater. The approach could find applications in shellfish hatcheries and depuration plants to ensure biosecurity requirements.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
English
Matèries (CDU)
574 - Ecologia general i biodiversitat
637 - Productes dels animals domèstics, de la caça i de la pesca
Pàgines
21
Publicat per
Elsevier
Publicat a
Aquaculture
Citació
Toldrà, Anna, Karl B. Andree, Ana Roque, Assaf Lowenthal, Ytzhak Rozenberg, M. Dolors Furones, and Mònica Campàs. 2021. "Use Of Anionic Polymer-Coated Magnetic Beads To Pre-Concentrate Ostreid Herpesvirus 1 From Seawater: Application To A UV Disinfection Treatment". Aquaculture 536: 736452. doi:10.1016/j.aquaculture.2021.736452.
Número de l'acord de la subvenció
EC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI
Programa
Aigües Marines i Continentals
Aqüicultura
Aquest element apareix en la col·lecció o col·leccions següent(s)
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