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dc.contributor.authorCarratalá, Jose V.
dc.contributor.authorBrouillette, Eric
dc.contributor.authorSerna, Naroa
dc.contributor.authorSánchez-Chardi, Alejandro
dc.contributor.authorSánchez, Julieta M.
dc.contributor.authorVillaverde, Antonio
dc.contributor.authorArís, Anna
dc.contributor.authorGarcia-Fruitós, Elena
dc.contributor.authorFerrer-Miralles, Neus
dc.contributor.authorMalouin, François
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-02-25T10:13:45Z
dc.date.available2021-02-25T10:13:45Z
dc.date.issued2020-12-17
dc.identifier.citationCarratalá, Jose V., Eric Brouillette, Naroa Serna, Alejandro Sánchez-Chardi, Julieta M. Sánchez, Antonio Villaverde, Anna Arís, Elena Garcia-Fruitós, Neus Ferrer-Miralles, and François Malouin. 2020. "In Vivo Bactericidal Efficacy Of GWH1 Antimicrobial Peptide Displayed On Protein Nanoparticles, A Potential Alternative To Antibiotics". Pharmaceutics 12 (12): 1217. doi:10.3390/pharmaceutics12121217.ca
dc.identifier.issn1999-4923ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1155
dc.description.abstractOligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to conventional antibiotics. In this work, the antimicrobial peptide, GWH1, was N-terminally fused to two different scaffold proteins, namely, GFP and IFN-γ for its bacterial production in the form of such recombinant protein complexes. Protein self-assembling as regular soluble protein nanoparticles was achieved in the case of GWH1-GFP, while oligomerization into bacterial inclusion bodies was reached in both constructions. Among all these types of therapeutic proteins, protein nanoparticles of GWH1-GFP showed the highest bactericidal effect in an in vitro assay against Escherichia coli, whereas non-oligomerized GWH1-GFP and GWH1-IFN-γ only displayed a moderate bactericidal activity. These results indicate that the biological activity of GWH1 is specifically enhanced in the form of regular multi-display configurations. Those in vitro observations were fully validated against a bacterial infection using a mouse mastitis model, in which the GWH1-GFP soluble nanoparticles were able to effectively reduce bacterial loads.ca
dc.format.extent16ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofPharmaceuticsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleIn Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibioticsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00064-C02-02/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-00064-C02-01/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3390/pharmaceutics12121217ca
dc.contributor.groupProducció de Remugantsca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/