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In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics
dc.contributor.author | Carratalá, Jose V. | |
dc.contributor.author | Brouillette, Eric | |
dc.contributor.author | Serna, Naroa | |
dc.contributor.author | Sánchez-Chardi, Alejandro | |
dc.contributor.author | Sánchez, Julieta M. | |
dc.contributor.author | Villaverde, Antonio | |
dc.contributor.author | Arís, Anna | |
dc.contributor.author | Garcia-Fruitós, Elena | |
dc.contributor.author | Ferrer-Miralles, Neus | |
dc.contributor.author | Malouin, François | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2021-02-25T10:13:45Z | |
dc.date.available | 2021-02-25T10:13:45Z | |
dc.date.issued | 2020-12-17 | |
dc.identifier.citation | Carratalá, Jose V., Eric Brouillette, Naroa Serna, Alejandro Sánchez-Chardi, Julieta M. Sánchez, Antonio Villaverde, Anna Arís, Elena Garcia-Fruitós, Neus Ferrer-Miralles, and François Malouin. 2020. "In Vivo Bactericidal Efficacy Of GWH1 Antimicrobial Peptide Displayed On Protein Nanoparticles, A Potential Alternative To Antibiotics". Pharmaceutics 12 (12): 1217. doi:10.3390/pharmaceutics12121217. | ca |
dc.identifier.issn | 1999-4923 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/1155 | |
dc.description.abstract | Oligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to conventional antibiotics. In this work, the antimicrobial peptide, GWH1, was N-terminally fused to two different scaffold proteins, namely, GFP and IFN-γ for its bacterial production in the form of such recombinant protein complexes. Protein self-assembling as regular soluble protein nanoparticles was achieved in the case of GWH1-GFP, while oligomerization into bacterial inclusion bodies was reached in both constructions. Among all these types of therapeutic proteins, protein nanoparticles of GWH1-GFP showed the highest bactericidal effect in an in vitro assay against Escherichia coli, whereas non-oligomerized GWH1-GFP and GWH1-IFN-γ only displayed a moderate bactericidal activity. These results indicate that the biological activity of GWH1 is specifically enhanced in the form of regular multi-display configurations. Those in vitro observations were fully validated against a bacterial infection using a mouse mastitis model, in which the GWH1-GFP soluble nanoparticles were able to effectively reduce bacterial loads. | ca |
dc.format.extent | 16 | ca |
dc.language.iso | eng | ca |
dc.publisher | MDPI | ca |
dc.relation.ispartof | Pharmaceutics | ca |
dc.rights | Attribution 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00064-C02-02/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/ | ca |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-00064-C02-01/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/ | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.3390/pharmaceutics12121217 | ca |
dc.contributor.group | Producció de Remugants | ca |
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