Diet, Immunity, and Microbiota Interactions: An Integrative Analysis of the Intestine Transcriptional Response and Microbiota Modulation in Gilthead Seabream (Sparus aurata) Fed an Essential Oils-Based Functional Diet
Firmino, Joana P.
Balebona, M. Carmen
Cerezo, Isabel M.
Moriñigo, Miguel Ángel
Reyes-López, Felipe E.
Essential oils (EOs) are promising alternatives to chemotherapeutics in animal production due to their immunostimulant, antimicrobial, and antioxidant properties, without associated environmental or hazardous side effects. In the present study, the modulation of the transcriptional immune response (microarray analysis) and microbiota [16S Ribosomal RNA (rRNA) sequencing] in the intestine of the euryhaline fish gilthead seabream (Sparus aurata) fed a dietary supplementation of garlic, carvacrol, and thymol EOs was evaluated. The transcriptomic functional analysis showed the regulation of genes related to processes of proteolysis and inflammatory modulation, immunity, transport and secretion, response to cyclic compounds, symbiosis, and RNA metabolism in fish fed the EOs-supplemented diet. Particularly, the activation of leukocytes, such as acidophilic granulocytes, was suggested to be the primary actors of the innate immune response promoted by the tested functional feed additive in the gut. Fish growth performance and gut microbiota alpha diversity indices were not affected, while dietary EOs promoted alterations in bacterial abundances in terms of phylum, class, and genus. Subtle, but significant alterations in microbiota composition, such as the decrease in Bacteroidia and Clostridia classes, were suggested to participate in the modulation of the intestine transcriptional immune profile observed in fish fed the EOs diet. Moreover, regarding microbiota functionality, increased bacterial sequences associated with glutathione and lipid metabolisms, among others, detected in fish fed the EOs supported the metabolic alterations suggested to potentially affect the observed immune-related transcriptional response. The overall results indicated that the tested dietary EOs may promote intestinal local immunity through the impact of the EOs on the host-microbial co-metabolism and consequent regulation of significant biological processes, evidencing the crosstalk between gut and microbiota in the inflammatory regulation upon administration of immunostimulant feed additives.
639 - Caça. Pesca. Piscicultura
Is part of
Frontiers in Immunology
Firmino, Joana P., Eva Vallejos-Vidal, M. Carmen Balebona, Yuliaxis Ramayo-Caldas, Isabel M. Cerezo, Ricardo Salomón, and Lluis Tort et al. 2021. "Diet, Immunity, And Microbiota Interactions: An Integrative Analysis Of The Intestine Transcriptional Response And Microbiota Modulation In Gilthead Seabream (Sparus Aurata) Fed An Essential Oils-Based Functional Diet". Frontiers In Immunology 12. doi:10.3389/fimmu.2021.625297.
Grant agreement number
MAPAMA/JACUMAR/ /ES/Estrategias nutricionales para la mejora en el rendimiento productivo: uso de piensos funcionales y dietas salud en acuicultura/DIETAplus
MICINN/Programa Estatal de I+D+i orientada a los Retos de la sociedad, en el marco del Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095653-R-I00/ES/La dieta y microbiota intestinal como herramientas para regular la adiposidad en peces/ADIPOQUIZ
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/117RT0521/ES/Estrategias de desarrollo y mejora de la producción de larvas de peces en Iberoamerica/LARVAplus
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