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dc.contributor.authorArgüello, Héctor
dc.contributor.authorRodríguez‐Gómez, Irene Magdalena
dc.contributor.authorSánchez‐Carvajal, Jose María
dc.contributor.authorPallares, Francisco José
dc.contributor.authorDíaz, Iván
dc.contributor.authorCabrera‐Rubio, Raúl
dc.contributor.authorCrispie, Fiona
dc.contributor.authorCotter, Paul D.
dc.contributor.authorMateu, Enric
dc.contributor.authorMartín‐Valls, Gerard
dc.contributor.authorCarrasco, Librado
dc.contributor.authorGómez‐Laguna, Jaime
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-03-11T16:32:35Z
dc.date.available2021-03-11T16:32:35Z
dc.date.issued2021-03-03
dc.identifier.citationArgüello, Héctor, Irene Magdalena Rodríguez‐Gómez, Jose María Sánchez‐Carvajal, Francisco José Pallares, Iván Díaz, Raúl Cabrera‐Rubio, and Fiona Crispie et al. 2021. "Porcine Reproductive And Respiratory Syndrome Virus Impacts On Gut Microbiome In A Strain Virulence‐Dependent Fashion". Microbial Biotechnology. doi:10.1111/1751-7915.13757.ca
dc.identifier.issn1751-7915ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1188
dc.description.abstractPorcine reproductive and respiratory syndrome (PRRS) is a viral disease defined by reproductive problems, respiratory distress and a negative impact on growth rate and general condition. Virulent PRRS virus (PRRSV) strains have emerged in the last years with evident knowledge gaps in their impact on the host immune response. Thus, the present study examines the impact of acute PRRS virus (PRRSV) infection, with two strains of different virulence, on selected immune parameters and on the gut microbiota composition of infected pigs using 16S rRNA compositional sequencing. Pigs were infected with a low virulent (PRRS_3249) or a virulent (Lena) PRRSV‐1 strain and euthanized at 1, 3, 6, 8 or 13 days post‐inoculation (dpi). Faeces were collected from each animal at the necropsy time‐point. Alpha and beta diversity analyses demonstrated that infection, particularly with the Lena strain, impacted the microbiome composition from 6 dpi onwards. Taxonomic differences revealed that infected pigs had higher abundance of Treponema and Methanobrevibacter (FDR < 0.05). Differences were more considerable for Lena‐ than for PRRS_3249‐infected pigs, showing the impact of strain virulence in the intestinal changes. Lena‐infected pigs had reduced abundancies of anaerobic commensals such as Roseburia, Anaerostipes, Butyricicoccus and Prevotella (P < 0.05). The depletion of these desirable commensals was significantly correlated to infection severity measured by viraemia, clinical signs, lung lesions and immune parameters (IL‐6, IFN‐γ and Hp serum levels). Altogether, the results from this study demonstrate the indirect impact of PRRSV infection on gut microbiome composition in a strain virulence‐dependent fashion and its association with selected immune markers.ca
dc.format.extent10ca
dc.language.isoengca
dc.publisherWiley Open Accessca
dc.relation.ispartofMicrobial Biotechnologyca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlePorcine reproductive and respiratory syndrome virus impacts on gut microbiome in a strain virulence‐dependent fashionca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2014-16735/ES/ /ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-76111-R/ES/Caracterización del transcriptoma del macrófago alveolar porcino en las fases tempranas de la infección in vivo con cepas del virus del PRRS-1 de distinta virulencia/ca
dc.relation.projectIDEC/H2020/818368/EU/Microbiome Applications for Sustainable food systems through Technologies and EnteRprise/MASTERca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1111/1751-7915.13757ca
dc.contributor.groupSanitat Animalca


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