Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life
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Author
Rimet, Frédéric
Aylagas, Eva
Borja, Angel
Bouchez, Agnès
Canino, Alexis
Chauvin, Christian
Chonova, Teofana
Ciampor Jr, Fedor
Costa, Filipe O.
Ferrari, Benoit J.D.
Gastineau, Romain
Goulon, Chloé
Gugger, Muriel
Holzmann, Maria
Jahn, Regine
Kahlert, Maria
Kusbert, Wolf-Henning
Laplace-Treyture, Christophe
Leese, Florian
Leliaert, Frederik
Mann, David G.
Marchand, Frédéric
Méléder, Vona
Pawlowski, Jan
Rasconi, Serena
Rivera, Sinziana
Rougerie, Rodolphe
Schweizer, Magali
Vasselon, Valentin
Vivien, Régis
Weigand, Alexander
Witkowski, Andrzej
Zimmermann, Jonas
Ekrem, Torbjørn
Publication date
2021-02-26ISSN
2534-9708
Abstract
DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
17
Publisher
Pensoft Publishers
Is part of
Metabarcoding and Metagenomics (MBMG)
Citation
Rimet, Frédéric, Eva Aylagas, Ángel Borja, Agnès Bouchez, Alexis Canino, Christian Chauvin, and Teofana Chonova et al. 2021. "Metadata Standards And Practical Guidelines For Specimen And DNA Curation When Building Barcode Reference Libraries For Aquatic Life". Metabarcoding And Metagenomics 5. doi:10.3897/mbmg.5.58056.
Grant agreement number
EC/COST/CA15219/EU/Developing new genetic tools for bioassessment of aquatic ecosystems in Europe/DNAqua-Net
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2829]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/