Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach
Navas, María Jesús
Salas, Maria Luisa
The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8+ T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.
619 - Veterinary science
Is part of
Bosch-Camós, Laia, Elisabet López, María Jesús Navas, Sonia Pina-Pedrero, Francesc Accensi, Florencia Correa-Fiz, and Chankyu Park et al. 2021. "Identification Of Promiscuous African Swine Fever Virus T-Cell Determinants Using A Multiple Technical Approach". Vaccines 9 (1): 29. doi:10.3390/vaccines9010029.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la Sociedad/AGL2016-78160-C2-1-R/ES/ESTRATEGIAS DE PROTECCION FRENTE A LA PESTE PORCINA AFRICANA: DE LA INVESTIGACION BASICA A LA APLICADA/
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-107616RB-I00/ES/Peste porcina africana; de la emergencia a evitar el endemismo/
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