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dc.contributor.authorBosch-Camós, Laia
dc.contributor.authorLópez, Elisabet
dc.contributor.authorNavas, María Jesús
dc.contributor.authorPina-Pedrero, Sonia
dc.contributor.authorAccensi, Francesc
dc.contributor.authorCorrea-Fiz, Florencia
dc.contributor.authorPark, Chankyu
dc.contributor.authorCarrascal, Montserrat
dc.contributor.authorDomínguez, Javier
dc.contributor.authorSalas, Maria Luisa
dc.contributor.authorNikolin, Veljko
dc.contributor.authorCollado, Javier
dc.contributor.authorRodríguez, Fernando
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-03-17T12:09:11Z
dc.date.available2021-03-17T12:09:11Z
dc.date.issued2021-01-07
dc.identifier.citationBosch-Camós, Laia, Elisabet López, María Jesús Navas, Sonia Pina-Pedrero, Francesc Accensi, Florencia Correa-Fiz, and Chankyu Park et al. 2021. "Identification Of Promiscuous African Swine Fever Virus T-Cell Determinants Using A Multiple Technical Approach". Vaccines 9 (1): 29. doi:10.3390/vaccines9010029.ca
dc.identifier.issn2076-393Xca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1198
dc.description.abstractThe development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8+ T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.ca
dc.format.extent20ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofVaccinesca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleIdentification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approachca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la Sociedad/AGL2016-78160-C2-1-R/ES/ESTRATEGIAS DE PROTECCION FRENTE A LA PESTE PORCINA AFRICANA: DE LA INVESTIGACION BASICA A LA APLICADA/ca
dc.relation.projectIDMICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/PID2019-107616RB-I00/ES/ /ca
dc.relation.projectIDMICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-107616RB-I00/ES/ /ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3390/vaccines9010029ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/