Mostrar el registro sencillo del ítem
Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
dc.contributor.author | Riera-Heredia, Natàlia | |
dc.contributor.author | Martins, Rute | |
dc.contributor.author | Mateus, Ana Patrícia | |
dc.contributor.author | Costa, Rita A. | |
dc.contributor.author | Gisbert, Enric | |
dc.contributor.author | Navarro, Isabel | |
dc.contributor.author | Gutiérrez, Joaquim | |
dc.contributor.author | Power, Deborah M. | |
dc.contributor.author | Capilla, Encarnación | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2018-12-21T10:46:16Z | |
dc.date.available | 2018-12-21T10:46:16Z | |
dc.date.issued | 2018-07-25 | |
dc.identifier.citation | Riera-Heredia, Natàlia, Rute Martins, Ana Patrícia Mateus, Rita A. Costa, Enric Gisbert, Isabel Navarro, Joaquim Gutiérrez, Deborah M. Power, and Encarnación Capilla. 2018. "Temperature Responsiveness Of Gilthead Sea Bream Bone; An In Vitro And In Vivo Approach". Scientific Reports 8 (1). Springer Nature. doi:10.1038/s41598-018-29570-9. | ca |
dc.identifier.issn | 2045-2322 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/131 | |
dc.description.abstract | This study aimed to characterize the molecules involved in osteogenesis in seabream and establish using in vitro/in vivo approaches the responsiveness of selected key genes to temperature. The impact of a temperature drop from 23 to 13 °C was evaluated in juvenile fish thermally imprinted during embryogenesis. Both, in vitro/in vivo, Fib1a, appeared important in the first stages of bone formation, and Col1A1, ON and OP, in regulating matrix production and mineralization. OCN mRNA levels were up-regulated in the final larval stages when mineralization was more intense. Moreover, temperature-dependent differential gene expression was observed, with lower transcript levels in the larvae at 18 °C relative to those at 22 °C, suggesting bone formation was enhanced in the latter group. Results revealed that thermal imprinting affected the long-term regulation of osteogenesis. Specifically, juveniles under the low and low-to-high-temperature regimes had reduced levels of OCN when challenged, indicative of impaired bone development. In contrast, gene expression in fish from the high and high-to-low-temperature treatments was unchanged, suggesting imprinting may have a protective effect. Overall, the present study revealed that thermal imprinting modulates bone development in seabream larvae, and demonstrated the utility of the in vitro MSC culture as a reliable tool to investigate fish osteogenesis. | ca |
dc.format.extent | 14 | ca |
dc.language.iso | eng | ca |
dc.publisher | Springer Nature | ca |
dc.relation.ispartof | Scientific Reports | ca |
dc.rights | Attribution 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-17324/ES/CARACTERIZACION DE MOLECULAS CLAVE INVOLUCRADAS EN LA OSTEOBLASTOGENESIS, EL DESARROLLO OSEO Y LA APARICION DE DEFORMIDADES EN LA DORADA (SPARUS AURATA)/ | ca |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-57974-R/ES/ADIPOSIDAD Y METABOLISMO LIPIDICO EN PECES ALIMENTADOS CON DIETAS DE ACEITE DE PESCADO ALTAMENTE SUBSTITUIDO Y CRIADOS A ELEVADAS TEMPERATURAS: ENFOQUES IN VITRO E IN VIVO/ | ca |
dc.relation.projectID | EC/FP7/222719/EU/Building a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture/LIFECYCLE | ca |
dc.subject.udc | 639 | ca |
dc.identifier.doi | https://doi.org/10.1038/s41598-018-29570-9 | ca |
dc.contributor.group | Aqüicultura | ca |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
ARTICLES CIENTÍFICS [2.850]