Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
Mateus, Ana Patrícia
Costa, Rita A.
Power, Deborah M.
This study aimed to characterize the molecules involved in osteogenesis in seabream and establish using in vitro/in vivo approaches the responsiveness of selected key genes to temperature. The impact of a temperature drop from 23 to 13 °C was evaluated in juvenile fish thermally imprinted during embryogenesis. Both, in vitro/in vivo, Fib1a, appeared important in the first stages of bone formation, and Col1A1, ON and OP, in regulating matrix production and mineralization. OCN mRNA levels were up-regulated in the final larval stages when mineralization was more intense. Moreover, temperature-dependent differential gene expression was observed, with lower transcript levels in the larvae at 18 °C relative to those at 22 °C, suggesting bone formation was enhanced in the latter group. Results revealed that thermal imprinting affected the long-term regulation of osteogenesis. Specifically, juveniles under the low and low-to-high-temperature regimes had reduced levels of OCN when challenged, indicative of impaired bone development. In contrast, gene expression in fish from the high and high-to-low-temperature treatments was unchanged, suggesting imprinting may have a protective effect. Overall, the present study revealed that thermal imprinting modulates bone development in seabream larvae, and demonstrated the utility of the in vitro MSC culture as a reliable tool to investigate fish osteogenesis.
639 - Caça. Pesca. Piscicultura
Is part of
Riera-Heredia, Natàlia, Rute Martins, Ana Patrícia Mateus, Rita A. Costa, Enric Gisbert, Isabel Navarro, Joaquim Gutiérrez, Deborah M. Power, and Encarnación Capilla. 2018. "Temperature Responsiveness Of Gilthead Sea Bream Bone; An In Vitro And In Vivo Approach". Scientific Reports 8 (1). Springer Nature. doi:10.1038/s41598-018-29570-9.
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-17324/ES/CARACTERIZACION DE MOLECULAS CLAVE INVOLUCRADAS EN LA OSTEOBLASTOGENESIS, EL DESARROLLO OSEO Y LA APARICION DE DEFORMIDADES EN LA DORADA (SPARUS AURATA)/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-57974-R/ES/ADIPOSIDAD Y METABOLISMO LIPIDICO EN PECES ALIMENTADOS CON DIETAS DE ACEITE DE PESCADO ALTAMENTE SUBSTITUIDO Y CRIADOS A ELEVADAS TEMPERATURAS: ENFOQUES IN VITRO E IN VIVO/
EC/FP7/222719/EU/Building a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture/LIFECYCLE
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