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dc.contributor.authorRiera-Heredia, Natàlia
dc.contributor.authorMartins, Rute
dc.contributor.authorMateus, Ana Patrícia
dc.contributor.authorCosta, Rita A.
dc.contributor.authorGisbert, Enric
dc.contributor.authorNavarro, Isabel
dc.contributor.authorGutiérrez, Joaquim
dc.contributor.authorPower, Deborah M.
dc.contributor.authorCapilla, Encarnación
dc.contributor.otherProducció Animalca
dc.date.accessioned2018-12-21T10:46:16Z
dc.date.available2018-12-21T10:46:16Z
dc.date.issued2018-07-25
dc.identifier.citationRiera-Heredia, Natàlia, Rute Martins, Ana Patrícia Mateus, Rita A. Costa, Enric Gisbert, Isabel Navarro, Joaquim Gutiérrez, Deborah M. Power, and Encarnación Capilla. 2018. "Temperature Responsiveness Of Gilthead Sea Bream Bone; An In Vitro And In Vivo Approach". Scientific Reports 8 (1). Springer Nature. doi:10.1038/s41598-018-29570-9.ca
dc.identifier.issn2045-2322ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/131
dc.description.abstractThis study aimed to characterize the molecules involved in osteogenesis in seabream and establish using in vitro/in vivo approaches the responsiveness of selected key genes to temperature. The impact of a temperature drop from 23 to 13 °C was evaluated in juvenile fish thermally imprinted during embryogenesis. Both, in vitro/in vivo, Fib1a, appeared important in the first stages of bone formation, and Col1A1, ON and OP, in regulating matrix production and mineralization. OCN mRNA levels were up-regulated in the final larval stages when mineralization was more intense. Moreover, temperature-dependent differential gene expression was observed, with lower transcript levels in the larvae at 18 °C relative to those at 22 °C, suggesting bone formation was enhanced in the latter group. Results revealed that thermal imprinting affected the long-term regulation of osteogenesis. Specifically, juveniles under the low and low-to-high-temperature regimes had reduced levels of OCN when challenged, indicative of impaired bone development. In contrast, gene expression in fish from the high and high-to-low-temperature treatments was unchanged, suggesting imprinting may have a protective effect. Overall, the present study revealed that thermal imprinting modulates bone development in seabream larvae, and demonstrated the utility of the in vitro MSC culture as a reliable tool to investigate fish osteogenesis.ca
dc.format.extent14ca
dc.language.isoengca
dc.publisherSpringer Natureca
dc.relation.ispartofScientific Reportsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleTemperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approachca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-17324/ES/CARACTERIZACION DE MOLECULAS CLAVE INVOLUCRADAS EN LA OSTEOBLASTOGENESIS, EL DESARROLLO OSEO Y LA APARICION DE DEFORMIDADES EN LA DORADA (SPARUS AURATA)/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-57974-R/ES/ADIPOSIDAD Y METABOLISMO LIPIDICO EN PECES ALIMENTADOS CON DIETAS DE ACEITE DE PESCADO ALTAMENTE SUBSTITUIDO Y CRIADOS A ELEVADAS TEMPERATURAS: ENFOQUES IN VITRO E IN VIVO/ca
dc.relation.projectIDEC/FP7/222719/EU/Building a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture/LIFECYCLEca
dc.subject.udc639 - Caça. Pesca. Pisciculturaca
dc.identifier.doihttps://doi.org/10.1038/s41598-018-29570-9ca
dc.contributor.groupAqüiculturaca


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